PLK5 Antibody

Images

 
Western Blot: PLK5 Antibody [NBP1-97306] - WB analysis of PLK5 in 1. human brain lysate and 2. mouse brain lysate. Molecular weight of PLK5 differs between human and mouse species.
Immunocytochemistry/ Immunofluorescence: PLK5 Antibody [NBP1-97306] - PLK5 antibody was tested in HepG2 cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).
Immunohistochemistry: PLK5 Antibody [NBP1-97306] - IHC analysis of PLK5 in mouse salivary gland using DAB with hematoxylin counterstain.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

PLK5 Antibody Summary

Immunogen
A synthetic peptide made to an internal portion of the human PLK5 protein (between residues 200-250) [UniProt Q496M5]
Localization
Nucleus, nucleolus. Cytoplasm.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PLK5
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Immunocytochemistry/Immunofluorescence 1:40
  • Immunohistochemistry 1:40-1:200
  • Immunohistochemistry-Paraffin 1:40-1:200
Application Notes
This PLK5 antibody is useful for Western Blot, Immunocytochemistry/Immunofluorescence, and IHC-paraffin embedded sections. In Western Blot, a band is seen ~50 kDa in human brain lysate and ~66 kDa in mouse brain lysate. Molecular weight of PLK5 differs between human and mouse species. In ICC/IF, nuclear staining was observed in HepG2 cells. In IHC-P, nuclear and cytoplasmic staining was seen in mouse salivary gland tissue. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

Reactivity Notes

Human and mouse.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Tris-Glycine (pH 7.5) and 0.15M NaCl
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for PLK5 Antibody

  • MGC118807
  • MGC118808
  • PLK5P
  • polo-like kinase 5
  • polo-like kinase 5, pseudogene
  • SgK384ps

Background

PLK5 (PLK5P, Polo-like kinase 5) is a polo box domain-only protein that is down-regulated in proliferating cells and accumulates in quiescent cells. PLK5 is significantly silenced in brain cancer and overexpression of PKL5 triggers apoptotic effects, both of which suggest that it may have a tumor suppressor function. PLK5 has also been theorized to be important to the proper formation of neurite processes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

View specific protocols for PLK5 Antibody (NBP1-97306): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP1-97306AF405
Alexa Fluor 488 Labeled NBP1-97306AF488
Alexa Fluor 647 Labeled NBP1-97306AF647
Alexa Fluor 700 Labeled NBP1-97306AF700
Biotin Labeled NBP1-97306B
DyLight 405 Labeled NBP1-97306V
DyLight 488 Labeled NBP1-97306G
DyLight 550 Labeled NBP1-97306R
DyLight 650 Labeled NBP1-97306C
FITC Labeled NBP1-97306F
HRP Labeled NBP1-97306H

Additional PLK5 Products

PLK5 NBP1-97306

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Bioinformatics

Gene Symbol PLK5
Entrez
Uniprot