PLK5 Antibody - BSA Free

Western Blot: PLK5 Antibody [NBP1-97306] - WB analysis of PLK5 in 1. human brain lysate and 2. mouse brain lysate. Molecular weight of PLK5 differs between human and mouse species.

Immunocytochemistry/Immunofluorescence: PLK5 Antibody [NBP1-97306] - PLK5 antibody was tested in HepG2 cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).

Immunohistochemistry: PLK5 Antibody [NBP1-97306] - IHC analysis of PLK5 in mouse salivary gland using DAB with hematoxylin counterstain.

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

PLK5 Antibody - BSA Free Summary

• Immunogen: A synthetic peptide made to an internal portion of the human PLK5 protein (between residues 200-250) [UniProt Q496M5]
• Localization: Nucleus, nucleolus. Cytoplasm.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PLK5
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:40
  • Immunohistochemistry 1:40-1:200
  • Immunohistochemistry-Paraffin 1:40-1:200
  • Western Blot 1:1000
• Application Notes: This PLK5 antibody is useful for Western Blot, Immunocytochemistry/Immunofluorescence, and IHC-paraffin embedded sections. In Western Blot, a band is seen ~50 kDa in human brain lysate and ~66 kDa in mouse brain lysate. Molecular weight of PLK5 differs between human and mouse species. In ICC/IF, nuclear staining was observed in HepG2 cells. In IHC-P, nuclear and cytoplasmic staining was seen in mouse salivary gland tissue. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Tris-Glycine (pH 7.5) and 0.15M NaCl
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PLK5 Antibody - BSA Free

• MGC118807
• MGC118808
• PLK5P
• polo-like kinase 5
• polo-like kinase 5, pseudogene
• SgK384ps

Background

PLK5 (PLK5P, Polo-like kinase 5) is a polo box domain-only protein that is down-regulated in proliferating cells and accumulates in quiescent cells. PLK5 is significantly silenced in brain cancer and overexpression of PKL5 triggers apoptotic effects, both of which suggest that it may have a tumor suppressor function. PLK5 has also been theorized to be important to the proper formation of neurite processes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PLK5
• Entrez:
  • 126520(Human)
• Uniprot:
  • Q496M5(Human)
  • Q4FZD7(Mouse)