Plexin B3 Antibody [Alexa Fluor® 750]

• Reactivity: Mu, Rt
• Applications: WB, Flow, IHC, CyTOF-ready
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 750

Plexin B3 Antibody [Alexa Fluor® 750] Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant mouse Plexin B3
  His25-Gln1235
  Accession # Q9QY40
• Specificity: Detects mouse and rat Plexin B3 in direct ELISAs and Western blots. In direct ELISAs, approximately 38% cross-reactivity with recombinant human (rh) Plexin B3 is observed, and less than 1% cross-reactivity with rhPlexin B2 is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry
  • Immunohistochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Plexin B3 Antibody [Alexa Fluor® 750]

• PLEXB3
• Plexin B3
• plexin-B3
• PLEXR
• PLXN6
• PLXNB3

Background

Plexin-B3 (also plexin-6) is a 260-270 kDa member of the plexin-B subfamily, plexin family of proteins. It is found on postnatal neurons and oligodendroglia, and appears to have opposing functions. In a context-dependent manner, plexin-B3 may block cell process extension and induce growth cone collapse by binding to Sema5. Conversely, it may engage in homophilic interactions in trans, promoting growth cone extension and axon elongation. The HGF receptor Met has been reported to complex with plexin-B3 in cis, and this interaction may contribute to its reported opposing effects. Mature mouse plexin-B3 is an 1868 amino acid (aa) type I transmembrane glycoprotein. It contains a 1211 aa extracellular domain (ECD) (aa 25-1892) plus a 636 aa cytoplasmic region. The ECD contains one Sema-domain (aa 25-451), two PSI domains (aa 454-646) and four IPT regions (aa 814-1224). There is one isoform variant that contains an alternative Met start site 10 aa upstream of the standard site. Based on human studies, proteolysis of mouse plexin-B3 likely occurs, generating 160 kDa and 140 kDa ECD fragments. Over aa 25-1235, mouse plexin-B3 shares 95% and 82% aa identity with rat and human plexin-B3, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.