Recombinant Human Pleiotrophin/PTN Animal-Free Protein

SDS-Page: Recombinant Human Pleiotrophin/PTN Animal-Free Protein [NBP2-76225] - 1 ug in each lane (-) non-reducing conditions and (+) reducing conditions in a 4-20% Tris-Glycine gel.

• Reactivity: Hu
• Applications: PAGE
• Format: Animal Free
• Concentration: LYOPH

Recombinant Human Pleiotrophin/PTN Animal-Free Protein Summary

• Description: An un-tagged recombinant protein corresponding to Human Pleiotrophin/PTN
  
  

  Source: E. coli

  Amino Acid Sequence: MGKKEKPEKK VKKSDCGEWQ WSVCVPTSGD CGLGTREGTR TGAECKQTMK TQRCKIPCNW KKQFGAECKY QFQAWGECDL NTALKTRTGS LKRALHNAEC QKTVTISKPC GKLTKPKPQA ESKKKKKEGK KQEKMLD

  Produced with no materials of animal or human origin using ANIMAL-FREE purification processes.

  Production and Purification

  • No animal-derived ingredients, No FBS
  • E. coli based production system
  • Animal-Free facility, fermentor, columns, filters, labware, lyophilization, and storage
  
  

  Quality Assurance

  • Endotoxin levels at <1 EUs/ug
  • Purity levels at 95% or greater
• Specificity: Pleiotrophin/PTN is a monomer, 15.4 kDa (137 aa)
• Preparation Method: Protein is derived from genetically modified E. coli.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: PTN
• Purity: >95%, by SDS-PAGE
• Endotoxin Note: Acceptance criteria for endotoxin levels are <1 EUs/ug protein (determined by Kinetic LAL Method).

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 15.4 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
• Buffer: Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 10 mM sodium phosphate, pH 7.5
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: >95%, by SDS-PAGE
• Reconstitution Instructions: Sterile water at 0.1 mg/mL

Notes

Lot Specific Information:
Given endotoxin and ED50 values are acceptance criteria standards. For lot specific values, please contact technical support.

Detailed Reconstitution:
Centrifuge vial before opening. Suspend the product by gently pipetting the recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots.

Expiration Date:
12 months from date of receipt when stored at -20C to-80C as supplied. 1 month when stored at 4C after reconstituting as directed. 3 months when stored at -20C to -80C after reconstituting as directed.

Alternate Names for Recombinant Human Pleiotrophin/PTN Animal-Free Protein

• HARP
• HBBM
• HB-GAM
• HBGF8
• HBNF
• HBNF1
• HBNF-1
• Heparin-binding brain mitogen
• HNGF-8
• NEGF1HBGF-8
• neurite growth-promoting factor 1
• neurite growth-promoting factor1)
• OSF-1
• Osteoblast-specific factor 1
• Pleiotrophin
• PTN

Background

Heparin binding mitogenic protein. Has neurite extension activity

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PTN