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PLA2R1 Antibody (12-6-5) [PerCP]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow
Clone
12-6-5
Clonality
Monoclonal
Host
Mouse
Conjugate
PerCP

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-50248AF350
Alexa Fluor 405
NBP2-50248AF405
Alexa Fluor 488
NBP2-50248AF488
Alexa Fluor 532
NBP2-50248AF532
Alexa Fluor 594
NBP2-50248AF594
Alexa Fluor 647
NBP2-50248AF647
Alexa Fluor 700
NBP2-50248AF700
Alexa Fluor 750
NBP2-50248AF750
Allophycocyanin
NBP2-50248APC
Allophycocyanin/Cy7
NBP2-50248APCCY7
Biotin
NBP2-50248B
DyLight 350
NBP2-50248UV
DyLight 405
NBP2-50248V
DyLight 488
NBP2-50248G
DyLight 550
NBP2-50248R
DyLight 594
NBP2-50248DL594
DyLight 650
NBP2-50248C
DyLight 680
NBP2-50248FR
DyLight 755
NBP2-50248IR
FITC
NBP2-50248F
HRP
NBP2-50248H
Janelia Fluor 549
NBP2-50248JF549
Janelia Fluor 646
NBP2-50248JF646
PE
NBP2-50248PE
PE/Atto594
NBP2-50248PEATT594
PE/Cy5.5
NBP2-50248PECY55
PE/Cy7
NBP2-50248PECY7
Unconjugated
NBP2-50248
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-50248
Azide and BSA Free
NBP2-80912
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PLA2R1 Antibody (12-6-5) [PerCP] Summary

Immunogen
Human PLA2R extracellular sequence N-C3 (containing domains N-terminal Cysteine rich-FibII-CTLD1-CTLD2-CTLD3). Genbank acc no. U17033; amino acids 20-663 Recombinant protein expressed in HEK 293-EBNA-1 cells
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
PLA2R1
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • Flow Cytometry
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Notes

This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for PLA2R1 Antibody (12-6-5) [PerCP]

  • 180 kDa secretory phospholipase A2 receptor
  • CLEC13C
  • CLEC13CC-type lectin domain family 13 member C
  • phospholipase A2 receptor 1, 180kDa
  • PLA2G1R
  • PLA2IR
  • PLA2R1
  • PLA2-RM-type receptor
  • PLA2Rphospholipase A2 receptor 1, 180kD
  • secretory phospholipase A2 receptor

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Western blot shows lysates of human pancreas tissue. PVDF Membrane was probed with 2 µg/mL of Human PLA2G1B Monoclonal Antibody (Catalog # MAB5018) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for PLA2G1B at approximately 17 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.
Species: Hu
Applications: WB, IP
PLA2G2A Antibody
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AF4925
Western blot shows lysates of mouse small intestine tissue. PVDF Membrane was probed with 0.5 µg/mL of Sheep Anti-Mouse PLA2G2A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4925) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for PLA2G2A at approximately 17 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
PLA2G2A was detected in perfusion fixed frozen sections of mouse intestine using Sheep Anti-Mouse PLA2G2A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4925) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm of epithelial cells in intestinal glands. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Simple Western lane view shows lysates of mouse small intestine tissue, loaded at 0.2 mg/mL. A specific band was detected for PLA2G2A at approximately 27 kDa (as indicated) using 5 µg/mL of Sheep Anti-Mouse PLA2G2A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4925) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the12-230 kDa separation system.
Species: Mu
Applications: WB, Simple Western, IHC
Phospholipase A2 X
Antibody (5G11)
H00008399-M01-100ug
Western Blot: Phospholipase A2 X Antibody (5G11) [H00008399-M01-100ug] - Detection against Immunogen (36.96 KDa).
Western Blot: Phospholipase A2 X Antibody (5G11) [H00008399-M01-100ug] - Analysis of PLA2G10 expression in transfected 293T cell line by PLA2G10 monoclonal antibody (M01), clone 5G11. Lane 1: PLA2G10 transfected lysate (Predicted MW: 18.2 KDa). Lane 2: Non-transfected lysate.
ELISA: Phospholipase A2 X Antibody (5G11) [H00008399-M01-100ug] - Detection limit for recombinant GST tagged PLA2G10 is 0.03 ng/ml as a capture antibody.
Species: Hu
Applications: WB, ELISA
Phospholipase B1
Antibody
H00151056-B01P
Western Blot: Phospholipase B1 Antibody [H00151056-B01P] - Analysis of PLB1 expression in transfected 293T cell line by PLB1 polyclonal antibody. Lane 1: PLB1 transfected lysate(46.53 KDa). Lane 2: Non-transfected lysate.
(1 Publication)
Species: Hu, Mu
Applications: WB
14-3-3 zeta
Antibody (818515) [Un ...
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Western blot shows lysates of SH‑SY5Y human neuroblastoma cell line,SK‑Mel‑28 human malignant melanoma cell line, NIH‑3T3 mouse embryonic fibroblast cell line, and C6 rat glioma cell line. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human14‑3‑3 zeta  Monoclonal Antibody (Catalog # MAB2669) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for14‑3‑3 zeta at approximately 27 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Simple Western lane view shows lysates of SH‑SY5Y human neuroblastoma cell line, loaded at 0.5 mg/mL. A specific band was detected for 14‑3‑3 zeta at approximately 34 kDa (as indicated) using 2 µg/mL of Mouse Anti-Human 14‑3‑3 zeta Monoclonal Antibody (Catalog # MAB2669). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
14‑3‑3 zeta  was detected in immersion fixed paraffin-embedded sections of human squamous cell carcinoma using Mouse Anti-Human 14‑3‑3 zeta Monoclonal Antibody (Catalog # MAB2669) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counter­stained with hematoxylin (blue). Specific staining was localized to nuclei and plasma membrane. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
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Applications: WB, Simple Western, IHC
ATP8A2 Antibody
NBP2-55197
Immunocytochemistry/Immunofluorescence: ATP8A2 Antibody [NBP2-55197] - Staining of human cell line PC-3 shows localization to plasma membrane.
Species: Hu
Applications: ICC/IF
TNF-alpha
[Unconjugated]
210-TA
1 µg/lane of Recombinant Human TNF-alpha was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa.
Recombinant Human TNF-alpha (Catalog # 210‑TA) induces cytotoxicity in the L-929 mouse fibroblast cell line in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is 25‑100 pg/mL.
(35 Reviews)
(649 Publications)
Species: Hu
Phospholipase A2
IID Antibody
NBP2-94062
Western Blot: Phospholipase A2 IID Antibody [NBP2-94062] - Analysis of extracts of various cell lines, using Phospholipase A2 IID at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
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H00008398-D01P
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alpha-Sarcoglycan
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NBP2-67150
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Immunohistochemistry-Frozen: alpha-Sarcoglycan Antibody (JA51-81) [NBP2-67150] - Human skeletal muscle tissue. IHC-Fr image submitted by a verified customer review.
Immunohistochemistry-Paraffin: alpha-Sarcoglycan Antibody (JA51-81) [NBP2-67150] - Analysis of paraffin-embedded mouse heart tissue using anti-alpha sarcoglycan antibody. Counter stained with hematoxylin.
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Species: Hu, Mu, Rt
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GPHA2 Antibody
NBP3-04883
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ATP6V0A2 Antibody
NBP1-59069
Western Blot: ATP6V0A2 Antibody [NBP1-59069] - HeLa cells, concentration 3.3 ug/ml.
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Neprilysin/CD10
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COX-2 Antibody
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IL-6 [HRP]
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Western Blot: PLA2G4A Antibody [NBP1-31344] - Sample (30 ug of whole cell lysate) A: Raji 7.5% SDS PAGE; antibody diluted at 1:1000.
Immunocytochemistry/Immunofluorescence: PLA2G4A Antibody [NBP1-31344] - Analysis of HeLa, using NBP1-31344 at 1:200 dilution.
Immunohistochemistry-Paraffin: PLA2G4A Antibody [NBP1-31344] - PLA2G4A antibody detects PLA2G4A protein at cytoplasm in mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. PLA2G4A antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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Western Blot: ERK1 Antibody (1E5) [NBP2-22203] - Western blot analysis of whole cell lysates from (1) MCF7 (2) NIH3T3 cell lines using ERK1 antibody (clone 1E5) at 1:1000 dilution. The signal was developed using HRP labeled goat-anti Mouse secondary antibody with ECL based detection.
Immunocytochemistry/Immunofluorescence: ERK1 Antibody (1E5) [NBP2-22203] - Analysis of NIH/3T3 cells using ERK1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Immunohistochemistry-Paraffin: ERK1 Antibody (1E5) [NBP2-22203] - Immunohistochemical analysis of paraffin-embedded breast cancer tissues using ERK1 mouse mAb with DAB staining.
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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-50248AF350
Alexa Fluor 405 NBP2-50248AF405
Alexa Fluor 488 NBP2-50248AF488
Alexa Fluor 532 NBP2-50248AF532
Alexa Fluor 594 NBP2-50248AF594
Alexa Fluor 647 NBP2-50248AF647
Alexa Fluor 700 NBP2-50248AF700
Alexa Fluor 750 NBP2-50248AF750
Allophycocyanin NBP2-50248APC
Allophycocyanin/Cy7 NBP2-50248APCCY7
Biotin NBP2-50248B
DyLight 350 NBP2-50248UV
DyLight 405 NBP2-50248V
DyLight 488 NBP2-50248G
DyLight 550 NBP2-50248R
DyLight 594 NBP2-50248DL594
DyLight 650 NBP2-50248C
DyLight 680 NBP2-50248FR
DyLight 755 NBP2-50248IR
FITC NBP2-50248F
HRP NBP2-50248H
Janelia Fluor 549 NBP2-50248JF549
Janelia Fluor 646 NBP2-50248JF646
PE NBP2-50248PE
PE/Atto594 NBP2-50248PEATT594
PE/Cy5.5 NBP2-50248PECY55
PE/Cy7 NBP2-50248PECY7
PerCP NBP2-50248PCP
Learn about Custom Antibody Labeling

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Bioinformatics

Gene Symbol PLA2R1