PLA2G4A Antibody

Images

 
Western Blot: PLA2G4A Antibody [NBP1-52050] - NBP1-52050 (1ug/ml) staining of HeLa lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

PLA2G4A Antibody Summary

Immunogen
Peptide with sequence C-QEKTFRQQRKEHIR corresponding to internal region according to NP_077734.1.
Epitope
C-QEKTFRQQRKEHIR
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
PLA2G4A
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 - 3 ug/ml
  • Peptide ELISA Detection limit 1:32000
Application Notes
WB: Approx. 90 kDa band observed in HeLa and NIH3T3 lysates (calculated MW of 85.2 kDa band according to NP_077734.1).
Publications
Read Publication using NBP1-52050.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Rat.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for PLA2G4A Antibody

  • calcium-dependent phospholipid-binding protein
  • cPLA2
  • cPLA2-alpha
  • lysophospholipase
  • MGC126350
  • phosphatidylcholine 2-acylhydrolase
  • Phospholipase A2 group IVA
  • phospholipase A2, group IVA (cytosolic, calcium-dependent)
  • PLA2G4A
  • PLA2G4cytosolic phospholipase A2

Background

HTATIP (HIV-1 Tat interacting protein TIP60, about 60kDa) belongs to the MYST family of histone acetyl transferases (HATs) and was originally isolated as an HIV-1 TAT-interactive protein. HATs play important roles in regulating chromatin remodeling, transcription and other nuclear processes by acetylating histone and nonhistone proteins. The nucleosome, made up of four core histone proteins (H2A, H2B, H3 and H4), is the primary building block of chromatin. In addition to the growing number of post-translational histone modifications regulating chromatin structure, cells can also exchange canonical histones with variant histones that can directly or indirectly modulate chromatin structure. There are five major variants of histone H2A: canonical H2A (most abundant), H2A.X, MacroH2A, H2ABbd and H2A.Z. Histone H2A.Z, the most conserved variant across species, functions as both a positive and negative regulator of transcription and is important for chromosome stability. Several homologous protein complexes, such as SWR-C, TIP60 and SRCAP (mammals), have been shown to catalyze the ATP-dependent exchange of H2A.Z for H2A in the nucleosome. This protein is a histone acetylase that has a role in DNA repair and apoptosis and is thought to play an important role in signal transduction.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

MAB5018
Species: Hu
Applications: WB, IP
AF4925
Species: Mu
Applications: WB, Simple Western, IHC
NBP1-81586
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-43581
Species: Hu
Applications: WB, ICC/IF, IP
MAB2669
Species: Hu
Applications: WB, Simple Western, IHC
NB100-689
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC, IHC-P
NBP1-32162
Species: Hu, Mu, Rt
Applications: WB, ICC/IF
NBP2-14101
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP2-22203
Species: Hu, Mu, Rt, Mk
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
AF5447
Species: Hu
Applications: WB, CyTOF-ready, ICFlow
H00008399-D01P
Species: Hu, Rt
Applications: WB
NBP1-89522
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-84168
Species: Hu
Applications: IHC, IHC-P
NBP2-37477
Species: Hu
Applications: WB, ELISA, ICC/IF, IHC

Publications for PLA2G4A Antibody (NBP1-52050)(1)

Reviews for PLA2G4A Antibody (NBP1-52050) (0)

There are no reviews for PLA2G4A Antibody (NBP1-52050). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for PLA2G4A Antibody (NBP1-52050). (Showing 1 - 1 of 1 FAQ).

  1. Hi. Actually I want to purchase anti cPLA2 antibody raised in rabbit which can bind to both phorphorylated and unphosphorylated mouse cPLA2 group IV. I want to a gel shift assay with western blotting to show that my protein of interest is causing the phosphorylation of cPLA2 inside the mouse macrophages RAW cells.Can you please tell me which antibody would be suitable for the purpose. Thanks a lot.
    • Here are our antibodies raised in rabbit that have been validated to detect cPLA2 in mouse samples in a western blot. We have not specifically confirmed the ability of these antibodies to detect both the phosphorylated and unphosphorylated forms of the protein, and I cannot guarantee that they will clearly differentiate between the two.

Secondary Antibodies

 

Isotype Controls

Additional PLA2G4A Products

Bioinformatics Tool for PLA2G4A Antibody (NBP1-52050)

Discover related pathways, diseases and genes to PLA2G4A Antibody (NBP1-52050). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for PLA2G4A Antibody (NBP1-52050)

Discover more about diseases related to PLA2G4A Antibody (NBP1-52050).
 

Pathways for PLA2G4A Antibody (NBP1-52050)

View related products by pathway.

PTMs for PLA2G4A Antibody (NBP1-52050)

Learn more about PTMs related to PLA2G4A Antibody (NBP1-52050).

Blogs on PLA2G4A

There are no specific blogs for PLA2G4A, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our PLA2G4A Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol PLA2G4A
Entrez
Uniprot