PITPNM3 Antibody

Immunohistochemistry-Paraffin: PITPNM3 Antibody [NB300-814] - IHC analysis of PITPNM3 in paraffin embedded Human Cerebral Cortex using NB300-814 at 5ug/ml. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu, Mu, Ca
• Applications: ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

PITPNM3 Antibody Summary

• Description: Novus Biologicals Goat PITPNM3 Antibody (NB300-814) is a polyclonal antibody validated for use in IHC and ELISA. Anti-PITPNM3 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-SWARGPPKFESVP corresponding to C-Terminus according to NP_112497.2, NP_001159438.1.
• Predicted Species: Mouse (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PITPNM3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5 - 10 ug/ml
  • Immunohistochemistry-Paraffin 5 - 10 ug/ml
  • Peptide ELISA Detection limit 1:16000
• Application Notes: WB: Preliminary experiments gave an approx. 50 kDa band in human heart lysates after 0.1 ug/ml antibody staining. The 50 kDa band was successfully blocked by incubation with the immunizing peptide. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 107 kDa band according to NP_112497. IHC-P: Human cerebral cortex shows strong staining of cytoplasm in several neuronal cells.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PITPNM3 Antibody

• membrane-associated phosphatidylinositol transfer protein 3
• MGC157740
• MGC157741
• NIR-1
• NIR1CORD5
• Phosphatidylinositol transfer protein, membrane-associated 3
• PITPnm 3
• PITPNM family member 3
• Pyk2 N-terminal domain-interacting receptor 1
• RDGBA3
• retinal degeneration B alpha 3

Background

NIR1 is calcium ion binding, has phosphatidylinositol transporter activity and receptor tyrosine kinase binding.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PITPNM3 Antibody (NB300-814)(1)

Publication using NB300-814

• Lev S, Hernandez J, Martinez R et al. Identification of a novel family of targets of PYK2 related to Drosophila retinal degeneration B (rdgB) protein. Mol Cell Biol 1999-03-01 [PMID: 10022914]

Bioinformatics

• Gene Symbol: PITPNM3
• Entrez:
  • 83394(Human)
• Uniprot:
  • Q9BZ71(Human)