Pin1 Antibody - BSA Free

Western Blot: Pin1 Antibody [NBP1-77757] - Lane 1: T9ug cells treated with scrambled (scr). Lane 2: T9ug cells treated with PIN1 kd1 shRNA. Lane 3: T9ug cells treated with PIN1-overexpressing plasmid HAP1. Lane 4: T9ug cells treated with PIN1-overexpressing plasmid HAP1S67E. Lane 5: T9ug cells treated with PIN1 kd2 shRNA. Load: 25 ug per lane. Primary antibody: PIN 1 antibody at 1:400 for overnight at 4C. Secondary antibody: IRDye800 (TM) rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted size: 18 kDa for PIN-1. Other band(s): normalized with a-tubulin (a-tub) antibody.

Immunohistochemistry: Pin1 Antibody [NBP1-77757] - Tissue: testis. Fixation: formalin fixed paraffin embedded. Primary antibody: Anti-PIN1 at 5 ug/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Staining: PIN-1 as precipitated red signal with hematoxylin purple nuclear counterstain.

Western Blot: Pin1 Antibody [NBP1-77757] - Detection of endogenous Pin1 in HeLa whole cell lysates. The sample was run in duplicate. A band representing Pin1 is indicated by the arrowhead. Cell lysates were electrophoresed using a straight 15% polyacrylamide gel, followed by transfer to nitrocellulose. The membrane was probed with the primary antibody at a 1:700 dilution. A 1:5,000 dilution of HRP Gt-a-Rabbit IgG was used with a 15 sec exposure time.

Western Blot: Pin1 Antibody [NBP1-77757] - Lane 1: T9ug cells incubated with GST-Pin1. Lane 2: T9ug cells incubated with GST-CDK4/cyclinD1. Lane 3: T9ug cells incubated with GST-Pin1 and GST-CDK4/cyclinD1. Immunoprecipitated with pRb antibody. Load: 25 ug per lane. Primary antibody: anti-GST 1:400 for overnight at 4C. Secondary antibody: IRDye800 (TM) secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C.

Immunohistochemistry of Pin1 Antibody. Tissue: testis. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Anti-PIN1 at 5 ug/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Staining: PIN-1 as precipitated red signal with hematoxylin purple nuclear counterstain.

• Reactivity: Hu
• Applications: WB, ELISA, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Pin1 Antibody - BSA Free Summary

• Description: This affinity purified antibody is directed against human Pin1. The product was affinity purified from monospecific antiserum by immunoaffinity chromatography
  
  Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
• Immunogen: This affinity purified Pin1 Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal sequence of human Pin1. (Uniprot: Q13526)
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PIN1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:2500-1:10000
  • Immunohistochemistry 5ug/ml
  • Immunoprecipitation 1:100
  • Western Blot 1:500-1:3000
• Application Notes: This affinity purified antibody has been tested for use in ELISA, Immunohistochemistry, and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 18 kDa in size corresponding to Pin1 by western blotting in the appropriate cell lysate or extract. Lysates from 3T3, Jurkat, 293 or HeLa cells, as well as HeLa nuclear extract, are recommended for use as positive controls.

Reactivity Notes

A BLAST analysis was used to suggest cross-reactivity with Pin1 from human, dog, bovine and monkey based on a 100% homology with the immunizing sequence. Expect partial reactivity with Pin1 from mouse and rat sources based on 92% sequence homologies. Reactivity against homologues from other sources is not known.

Packaging, Storage & Formulations

• Storage: Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Preservative: 0.01% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for Pin1 Antibody - BSA Free

• dod
• EC 5.2.1.8
• peptidylprolyl cis/trans isomerase, NIMA-interacting 1
• peptidyl-prolyl cis/trans isomerase, NIMA-interacting
• peptidyl-prolyl cis-trans isomerase NIMA-interacting 1
• Peptidyl-prolyl cis-trans isomerase Pin1
• Pin1
• PPIase Pin1
• prolyl isomerase
• protein (peptidyl-prolyl cis/trans isomerase) NIMA-interacting 1
• protein (peptidylprolyl cis/trans isomerase) NIMA-interacting 1
• Rotamase Pin1
• UBL5

Background

Pin1 (peptidylprolyl cis/trans isomerase NIMA-interacting 1 protein) is an essential peptidylprolyl isomerase that regulates mitosis, presumably by interacting with NIMA and attenuating its mitosis-promoting activity. Pin1 displays a preference for an acidic residue N-terminal to the isomerized proline bond and also catalyzes pSer/Thr-Pro cis/trans isomerizations. Pin1 shows a nuclear localization.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PIN1
• Uniprot:
  • Q13526()
  • Q13526()
  • Q13526()
  • Q13526()