Pin1 Antibody

Images

 
Western Blot: Pin1 Antibody [NBP1-77757] - Lane 1: T9ug cells treated with scrambled (scr). Lane 2: T9ug cells treated with PIN1 kd1 shRNA. Lane 3: T9ug cells treated with PIN1-overexpressing plasmid HAP1. Lane 4: T9ug ...read more
Immunohistochemistry: Pin1 Antibody [NBP1-77757] - Tissue: testis. Fixation: formalin fixed paraffin embedded. Primary antibody: Anti-PIN1 at 5 ug/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary ...read more
Western Blot: Pin1 Antibody [NBP1-77757] - Detection of endogenous Pin1 in HeLa whole cell lysates. The sample was run in duplicate. A band representing Pin1 is indicated by the arrowhead. Cell lysates were ...read more
Western Blot: Pin1 Antibody [NBP1-77757] - Lane 1: T9ug cells incubated with GST-Pin1. Lane 2: T9ug cells incubated with GST-CDK4/cyclinD1. Lane 3: T9ug cells incubated with GST-Pin1 and GST-CDK4/cyclinD1. ...read more

Product Details

Summary
Reactivity Hu, Bv, Ca, PmSpecies Glossary
Applications WB, ELISA, IHC, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Pin1 Antibody Summary

Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal sequence of human Pin1.
Specificity
A BLAST analysis was used to suggest cross-reactivity with Pin1 from human, dog, bovine and monkey based on a 100% homology with the immunizing sequence. Expect partial reactivity with Pin1 from mouse and rat sources based on 92% sequence homologies. Reactivity against homologues from other sources is not known.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PIN1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • ELISA 1:2500-1:10000
  • Immunohistochemistry 1:10-1:500
  • Immunoprecipitation 1:100
Application Notes
This affinity purified antibody has been tested for use in ELISA and western blotting. Expect a band approximately 18 kDa in size corresponding - Pin1 by western blotting in the appropriate cell lysate or extract. Lysates from 3T3, Jurkat, 293 or HeLa cells, as well as HeLa nuclear extract, are recommended for use as positive controls.

Reactivity Notes

Reactive in human, dog, bovine, monkey

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Pin1 Antibody

  • dod
  • EC 5.2.1.8
  • peptidylprolyl cis/trans isomerase, NIMA-interacting 1
  • peptidyl-prolyl cis/trans isomerase, NIMA-interacting
  • peptidyl-prolyl cis-trans isomerase NIMA-interacting 1
  • Peptidyl-prolyl cis-trans isomerase Pin1
  • Pin1
  • PPIase Pin1
  • prolyl isomerase
  • protein (peptidyl-prolyl cis/trans isomerase) NIMA-interacting 1
  • protein (peptidylprolyl cis/trans isomerase) NIMA-interacting 1
  • Rotamase Pin1
  • UBL5

Background

Pin1 (peptidylprolyl cis/trans isomerase NIMA-interacting 1 protein) is an essential peptidylprolyl isomerase that regulates mitosis, presumably by interacting with NIMA and attenuating its mitosis-promoting activity.  Pin1 displays a preference for an acidic residue N-terminal to the isomerized proline bond and also catalyzes pSer/Thr-Pro cis/trans isomerizations.  Pin1 shows a nuclear localization.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol PIN1
Entrez