PIEZO2 Antibody (1081503) [Unconjugated]

PIEZO2 was detected in immersion fixed paraffin-embedded sections of human breast using Mouse Anti-Human PIEZO2 Monoclonal Antibody (Catalog # MAB11559) at 0.05 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cell surface. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

• Reactivity: Hu
• Applications: IHC
• Clone: 1081503
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

PIEZO2 Antibody (1081503) [Unconjugated] Summary

• Immunogen: PIEZO2 containing synthetic peptide
  Accession # Q9H5I5
• Specificity: Detects a synthetic peptide specific for Piezo2 around amino acid 2115 in Direct ELISA.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 0.5-15 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Reconstitution Instructions: Reconstitute lyophilized material at 0.2mg/ml in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PIEZO2 Antibody (1081503) [Unconjugated]

• C18orf30
• C18orf58
• DA3
• DA5
• DAIPT
• FAM38B
• FAM38B2
• family with sequence similarity 38, member A pseud
• family with sequence similarity 38, member B
• family with sequence similarity 38, member B2
• FLJ23144
• FLJ23403
• FLJ25916
• FLJ34907
• FLJ37734
• FLJ45725
• HsT748
• HsT771
• MWKS
• PIEZO2
• piezo-type mechanosensitive ion channel component
• piezo-type mechanosensitive ion channel component 2
• protein PIEZO2

Background

PIEZO2 is an 318 kDa mechanosensitive nonselective cation channel, found in cell types that typically respond to physical touch. PIEZO2 is an essential mechanotransducer for touch, proprioception, and interoception.  PIEZO2 is important for aspects of breathing, bladder control, blood pressure regulation and many other processes since it is expressed in several classes of jugular and nodose ganglia neurons. Loss of function of PIEZO2 results in profound lack of proprioception and is diagnosed as a rare disease caused PIEZO2 deficiency syndrome.

1. Wu J, Lewis AH, Grandl J. Touch, Tension, and Transduction - The Function and Regulation of Piezo Ion Channels. Trends Biochem Sci. 2017 Jan;42(1):57-71. doi: 10.1016/j.tibs.2016.09.004. Epub 2016 Oct 12. PMID: 27743844; PMCID: PMC5407468. 
2. Szczot M, Nickolls AR, Lam RM, Chesler AT. The Form and Function of PIEZO2. Annu Rev Biochem. 2021 Jun 20;90:507-534. doi: 10.1146/annurev-biochem-081720-023244. PMID: 34153212; PMCID: PMC8794004.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for PIEZO2 Antibody (MAB11559). (Showing 1 - 1 of 1 FAQ).

1. In my project we need Anti Piezo2 and we find it in your catalogs. PIEZO2 Antibody. I want to know the prediction size of your antibody. The size of the protein is more than 200 kd. but in this photo there there are some sharp band are there the actual bands?
   • The predicted molecular weights for this protein are based on the multiple transcripts associated with this protein. Please see <a href=" http://may2012.archive.ensembl.org/Homo_sapiens/Gene/Summary?g=ENSG00000154864;r=18:10670238-11148761" target="_blank">Ensembl </a> for detailed information. Those expected molecular weights are the following: 318.1, 80.8, 73.8, 62.7 kDa. We see this kind of staining across many of our antibodies for PIEZO2. It would appear that the transcript present is dependent on the tissue type. Based on protein arrays, we do believe that this antibody is specific.

Bioinformatics

• Uniprot:
  • Q9H5I5(Human)