PHPT1 Antibody

Western Blot: PHPT1 Antibody [NBP2-78798] - Samples: Whole cell lysate (50 ug) from GaMG, RKO, and LNCaP cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PHPT1 antibody NBP2-78798 used for WB at 1:1000. Detection: Chemiluminescence with an exposure time of 10 seconds.

Product Discontinued

PHPT1 Antibody Summary

• Immunogen: between 1 and 50
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PHPT1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000

Packaging, Storage & Formulations

• Storage: Store at 2 - 8 C / 1 year from date of receipt
• Buffer: TBS containing 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Purity: Affinity purified

Alternate Names for PHPT1 Antibody

• bA216L13.10
• DKFZp564M173
• EC 3.1.3,14 kDa phosphohistidine phosphatase
• EC 3.1.3.-
• HSPC141
• phosphohistidine phosphatase 11700008C22Rik
• phosphohistidine phosphatase 14kDa
• PHP14CGI-202
• Protein janus-A homolog
• RP11-216L13.10
• sex-regulated protein janus-a

Background

PHPT1 is a 125 amino acid enzyme belonging to the Janus protein family. Existing as a monomer in the cytoplasm, PHPT1 is an EDTA-insensitive phosphohistidine phosphatase. Overexpression of PHPT1 leads to specific phosphohistidine phosphatase activity towards phosphopeptide I, with no activity detected towards phosphotyrosine, phosphothreonine and phosphoserine peptides. Recombinant human PHPT1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PHPT1
• Uniprot:
  • Q9NRX4(Human)