PHF8 Antibody

Western Blot: PHF8 Antibody [NBP2-76957] - Western blot analysis of PHF8 on different cell lysate using anti-PHF8 antibody at 1/500 dilution.Positive control:Lane1: PC-3MLane2: A431Lane3: Human kidney tissue

Immunocytochemistry/Immunofluorescence: PHF8 Antibody [NBP2-76957] - ICC staining PHF8 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry: PHF8 Antibody [NBP2-76957] - Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-PHF8 antibody. Counter stained with hematoxylin.

Flow Cytometry: PHF8 Antibody [NBP2-76957] - Flow cytometric analysis of SiHa cells with PHF8 antibody at 1/100 dilution (fuchsia) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

Immunocytochemistry/Immunofluorescence: PHF8 Antibody [NBP2-76957] - ICC staining PHF8 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: PHF8 Antibody [NBP2-76957] - ICC staining PHF8 in JAR cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry: PHF8 Antibody [NBP2-76957] - Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-PHF8 antibody. Counter stained with hematoxylin.

Immunohistochemistry: PHF8 Antibody [NBP2-76957] - Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-PHF8 antibody. Counter stained with hematoxylin.

Immunohistochemistry: PHF8 Antibody [NBP2-76957] - Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-PHF8 antibody. Counter stained with hematoxylin.

Product Discontinued

PHF8 Antibody Summary

• Immunogen: Recombinant protein within Human PHF8 aa 153-362 / 1,060. (SwissProt: Q9UPP1 Human)
• Localization: Nuclear membrane.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PHF8
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:2000
• Theoretical MW: 118 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH7.4), 0.2% BSA, 50% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PHF8 Antibody

• EC 1.14.11.27
• histone lysine demethylase PHF8
• jumonji C domain-containing histone demethylase 1F
• KIAA1111
• KIAA1111ZNF422JHDM1F
• MRXSSD
• PHD finger protein 8DKFZp686E0868
• PHF8
• ZNF422

Background

PHF8 (PHD finger protein 8) is a member of the jumonji family of proteins and contains a jumonji C (JmjC) domain. The JmjC proteins are predicted to be metalloenzymes that play a role a chromatin remodeling and histone demethylation. PHF8 also bears a PHD (plant homeodomain)- type zinc-finger, a domain also found to be involved in chromatin remodeling and transcriptional regulation. Truncating mutations in PHF8 are associated with X-linked mental retardation and cleft lip/cleft palate. PHF8 is also known as ZNF422 and KIAA1111.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PHF8