PER3 Antibody

Product Discontinued

PER3 Antibody Summary

• Immunogen: Synthetic peptide, corresponding to amino acids N-terminus of Human Per3.
• Specificity: Per3 (P2) detects endogenous levels of Per3 protein.
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PER3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:1000
• Application Notes: Western blot (WB) analysis of Per3 (P2) pAb in extracts from Jurkat cells treated with insulin 0.01U.
  Immunohistochemistry (IHC) analyzes of Per3 (P2) pAb in paraffin-embedded human breast carcinoma tissue.
• Theoretical MW: 43 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2)
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PER3 Antibody

• Cell growth-inhibiting gene 13 protein
• Circadian clock protein PERIOD 3
• GIG13
• growth-inhibiting protein 13
• hPER3
• period (Drosophila) homolog 3
• period 3
• period circadian protein 3
• period circadian protein homolog 3
• period homolog 3 (Drosophila)

Background

Biological timepieces called circadian clocks are responsible for the regulation of hormonal rhythms, sleep cycles and other behaviors. The superchiasmatic nucleus (SCN), which is located in the brain, was the first mammalian circadian clock to be discovered. A number of transcription factors appearing to be molecular components of the SCN clock have been identified. Mutations within the Clock gene increase the length of the endogenous period and cause a loss of rhythmicity of circadian oscillations. Three mammalian period proteins, designated Per1, Per2 and Per3, exhibit circadian rhythyms in the SCN.During subjective night, Per1 and Per2 RNA levels increase in response to light pulses while Per3 RNA levels show no change in response to light pulses. Tim, for timeless, interacts with Per1 as well as Per2; and Tim and Per1 negatively regulate Clock-BMAL1-induced transcription.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PER3
• Entrez:
  • 8863(Human)