PEBP4 Antibody

Western Blot: PEBP4 Antibody [NBP1-46155] - NBP1-46155 (1ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: PEBP4 Antibody [NBP1-46155] - Analysis in human Pancreas shows staining consistent with lysosomes.

• Reactivity: Hu
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

PEBP4 Antibody Summary

• Description: Novus Biologicals Goat PEBP4 Antibody (NBP1-46155) is a polyclonal antibody validated for use in IHC, WB and ELISA. Anti-PEBP4 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-RERASEPKHKNQAE corresponding to C-Terminus according to NP_659399.2.
• Epitope: C-terminus
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PEBP4
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5 - 10 ug/ml
  • Immunohistochemistry-Paraffin 5 - 10 ug/ml
  • Peptide ELISA Detection limit 1:4000
  • Western Blot 1 - 3 ug/ml
• Application Notes: WB: Approx. 26 kDa band observed in human heart lysates (calculated MW of 25.7 kDa band according to NP_659399.2). An additional band of unknown identity was also consistently observed at 30 kDa. This band was successfully blocked by incubation with the immunizing peptide. IHC-P: Human pancreas shows staining consistent with lysosomes.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PEBP4 Antibody

• CORK-1
• CORK1GWTM1933
• cousin-of-RKIP 1 protein
• hPEBP4PRO4408
• MGC22776
• PEBP-4
• PEPB4
• phosphatidylethanolamine-binding protein 4
• Protein cousin-of-RKIP 1

Background

The phosphatidylethanolamine (PE)-binding proteins, including PEBP4, are an evolutionarily conserved family of proteins with pivotal biologic functions, such as lipid binding and inhibition of serine proteases (Wang et al., 2004 [PubMed 15302887]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PEBP4 Antibody (NBP1-46155)(1)

Publication using NBP1-46155

• Trynka G, Zhernakova A, Romanos J et al. Coeliac disease associated risk variants in TNFAIP3 and REL implicate altered NF-{kappa}B signalling. Gut 2009-02-24 [PMID: 19240061]

Bioinformatics

• Gene Symbol: PEBP4
• Entrez:
  • 157310(Human)
• Uniprot:
  • Q96S96(Human)