PDK-1 Antibody

Western Blot: PDPK1 Antibody [H00005170-B01P] - Analysis of PDPK1 expression in K-562.

Western Blot: PDPK1 Antibody [H00005170-B01P] - expression in Raw 264.7.

Western Blot: PDPK1 Antibody [H00005170-B01P] - Analysis of PDPK1 expression in NIH/3T3.

Western Blot: PDPK1 Antibody [H00005170-B01P] - Analysis of PDPK1 expression in transfected 293T cell line by PDPK1 polyclonal antibody. Lane 1: PDPK1 transfected lysate(47.19 KDa). Lane 2: Non-transfected lysate.

Proximity Ligation Assay: PDPK1 Antibody [H00005170-B01P] - Analysis of protein-protein interactions between RPS6KA3 and PDPK1. HeLa cells were stained with anti-RPS6KA3 rabbit purified polyclonal 1:1200 and anti-PDPK1 mouse purified polyclonal antibody 1:50. Each red dot represents the detection of protein-protei

Product Discontinued

PDK-1 Antibody Summary

• Description: Quality control test: Antibody reactive against mammalian transfected lysate.
• Immunogen: PDPK1 (NP_112558.2, 1 a.a. - 429 a.a.) full-length human protein. MARTTSQLYDAVPIQSSVVLCSCPSPSMVRTQTESSTPPGIPGGSRQGPAMDGTAAEPRPGAGSLQHAQPPPQPRKKRPEDFKFGKILGEGSFSTVVLARELATSREYATRANSFVGTAQYVSPELLTEKSACKSSDLWALGCIIYQLVAGLPPFRAGNEYLIFQKIIKLEYDFPEKFFPKARDLVEKLLVLDATKRLGCEEMEGYGPLKAHPFFESVTWENLHQQTPPKLTAYLPAMSEDDEDCYGNYDNLLSQFGCMQVSSSSSSHSLSASDTGLPQRSGSNIEQYIHDLDSNSFELDLQFSEDEKRLLLEKQAGGNPWHQFVENNLILKMGPVDKRKGLFARRRQLLLTEGPHLYYVDPVNKVLKGEIPWSQELRPEAKNFKTFFVHTPNRTYYLMDPSGNAHKWCRKIQEVWRQRYQSHPDAAVQ
• Isotype: IgG
• Clonality: Polyclonal
• Host: Mouse
• Gene: PDPK1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Proximity Ligation Assay
  • Western Blot
• Application Notes: It has been used for WB.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: No Preservative
• Purity: Protein G purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for PDK-1 Antibody

• 3-phosphoinositide-dependent protein kinase 1
• 3-phosphoinositide-dependent protein kinase 2 pseudogene
• PDK1
• PDK-1
• PDPK1
• PDPK2
• PDPK2P
• PkB kinase like gene 1
• PKB kinase

Background

PDK-1 (3-phosphoinositide-dependent protein kinase, gene name PDPK1) is a 58 -68 kDa, 556 amino acid (aa) monomeric protein of the AGC serine/threonine kinase family. It is activated by phosphorylation in the presence of PtdIns(3,4,5) P3 or PtdIns(3,4) P2. Akt, S6 kinases, PKA and PKC-zeta are reported PDK-1 substrates. Through Akt, PDK-1 mediates many of the intracellular actions of insulin. Within the region used as an immunogen, human PDK-1 shares 98% aa identity with mouse and rat PDK-1. One reported isoform has an alternate start site at aa 50, while another lacking aa 238-263 is predicted to be catalytically inactive.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PDK-1 Antibody (H00005170-B01P)(1)

Publication using H00005170-B01P

• Liu CH, Chen TC, Chau GY et al. An analysis of protein-protein interactions in cross-talk pathways reveals CRKL as a novel prognostic marker in hepatocellular carcinoma. Mol Cell Proteomics 2013-02-08 [PMID: 23397142]

Bioinformatics

• Gene Symbol: PDPK1
• Uniprot:
  • NP_112558.2(Human)