PDK-1 Antibody (SD204-9)

Images

 
Western Blot: PDK-1 Antibody (SD204-9) [NBP2-67755] - Analysis of PDPK1 on different lysates using anti-PDPK1 antibody at 1/1,000 dilution. Positive control: Lane 1: 293T Lane 2: PC-12 Lane 3: HL-60
Immunocytochemistry/ Immunofluorescence: PDK-1 Antibody (SD204-9) [NBP2-67755] - Staining PDPK1 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with ...read more
Immunohistochemistry-Paraffin: PDK-1 Antibody (SD204-9) [NBP2-67755] - Analysis of paraffin-embedded mouse kidney tissue using anti-PDPK1 antibody. Counter stained with hematoxylin.
Immunocytochemistry/ Immunofluorescence: PDK-1 Antibody (SD204-9) [NBP2-67755] - Staining PDPK1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with ...read more
Immunocytochemistry/ Immunofluorescence: PDK-1 Antibody (SD204-9) [NBP2-67755] - Staining PDPK1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with ...read more
Immunohistochemistry-Paraffin: PDK-1 Antibody (SD204-9) [NBP2-67755] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-PDPK1 antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: PDK-1 Antibody (SD204-9) [NBP2-67755] - Analysis of paraffin-embedded human colon cancer tissue using anti-PDPK1 antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: PDK-1 Antibody (SD204-9) [NBP2-67755] - Analysis of paraffin-embedded human kidney tissue using anti-PDPK1 antibody. Counter stained with hematoxylin.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clone
SD204-9
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

PDK-1 Antibody (SD204-9) Summary

Additional Information
Recombinant Monoclonal Antibody.
Immunogen
N terminal human PDPK1
Localization
Cytoplasm, Nucleus, Cell membrane, Cell junction.
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
PDPK1
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:2000
  • Immunocytochemistry/Immunofluorescence 1:100-1:500
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin 1:50-1:200

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
TBS (pH7.4), 1%BSA, 40%Glycerol
Preservative
0.05% Sodium Azide
Purity
Protein A purified

Alternate Names for PDK-1 Antibody (SD204-9)

  • 3-phosphoinositide-dependent protein kinase 1
  • 3-phosphoinositide-dependent protein kinase 2 pseudogene
  • PDK1
  • PDK-1
  • PDPK1
  • PDPK2
  • PDPK2P
  • PkB kinase like gene 1
  • PKB kinase

Background

PDK-1 (3-phosphoinositide-dependent protein kinase, gene name PDPK1) is a 58 -68 kDa, 556 amino acid (aa) monomeric protein of the AGC serine/threonine kinase family. It is activated by phosphorylation in the presence of PtdIns(3,4,5) P3 or PtdIns(3,4) P2. Akt, S6 kinases, PKA and PKC-zeta are reported PDK-1 substrates. Through Akt, PDK-1 mediates many of the intracellular actions of insulin. Within the region used as an immunogen, human PDK-1 shares 98% aa identity with mouse and rat PDK-1. One reported isoform has an alternate start site at aa 50, while another lacking aa 238-263 is predicted to be catalytically inactive.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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