PDK-1 Antibody (SD204-9) Summary
Additional Information |
Recombinant Monoclonal Antibody. |
Immunogen |
Synthetic peptide within human PDK-1 aa 1-50. (SwissProt: O15530 Human;SwissProt: Q9Z2A0 Mouse;SwissProt: O55173 Rat) |
Localization |
Cell membrane, Nucleus, Cytoplasm, focal adhesion. |
Isotype |
IgG |
Clonality |
Monoclonal |
Host |
Rabbit |
Gene |
PDPK1 |
Purity |
Protein A purified |
Innovator's Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase. |
Applications/Dilutions
Dilutions |
- Immunocytochemistry/Immunofluorescence 1:100-1:500
- Immunohistochemistry
- Immunohistochemistry-Paraffin 1:50-1:200
- Western Blot 1:500-1:2000
|
Packaging, Storage & Formulations
Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
Buffer |
TBS (pH7.4), 1%BSA, 40%Glycerol |
Preservative |
0.05% Sodium Azide |
Concentration |
1 mg/ml |
Purity |
Protein A purified |
Alternate Names for PDK-1 Antibody (SD204-9)
Background
PDK-1 (3-phosphoinositide-dependent protein kinase, gene name PDPK1) is a 58 -68 kDa, 556 amino acid (aa) monomeric protein of the AGC serine/threonine kinase family. It is activated by phosphorylation in the presence of PtdIns(3,4,5) P3 or PtdIns(3,4) P2. Akt, S6 kinases, PKA and PKC-zeta are reported PDK-1 substrates. Through Akt, PDK-1 mediates many of the intracellular actions of insulin. Within the region used as an immunogen, human PDK-1 shares 98% aa identity with mouse and rat PDK-1. One reported isoform has an alternate start site at aa 50, while another lacking aa 238-263 is predicted to be catalytically inactive.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
guaranteed for 1 year from date of receipt.
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FAQs for PDK-1 Antibody (NBP2-67755) (0)
Secondary Antibodies
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Isotype Controls
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