PDE3A Antibody

Western Blot: PDE3A Antibody [NB300-673] - Western blot detection of PDE3A at an antibody dilution of 1:250.

• Reactivity: Hu, Mu, Rt, Ca, Pm, Xp
• Applications: WB, ELISA, IP
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

PDE3A Antibody Summary

• Description: Novus Biologicals Goat PDE3A Antibody (NB300-673) is a polyclonal antibody validated for use in WB, ELISA and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Synthetic peptide (selective for only PDE3A variant).
• Localization: Cell Membrane
• Specificity: Does not cross reacts with PDE4B variants nor with any other PDEs.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PDE3A
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunoprecipitation 1:200
  • Western Blot 1:500
• Application Notes: Immunoprecipitation and Western Blot. Detects two protein bands of approximately 60-80 kDa. Optimal dilutions/concentrations should be determined by the end user.
• Control:

  Jurkat Whole Cell Lysate
  PDE3A Overexpression Lysate

Reactivity Notes

Cross-reacts with Human, Mouse, Rat and Dog.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris/Glycine buffer, pH 7.4-7.8, HEPES,BSA 0.5%, glycerol 30%.
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PDE3A Antibody

• CGI-PDE A
• cGI-PDE
• cGMP-inhibited 3'-5'-cyclic phosphodiesterase A
• cyclic GMP inhibited phosphodiesterase A
• Cyclic GMP-inhibited phosphodiesterase A
• EC 3.1.4
• EC 3.1.4.17
• PDE3A
• phosphodiesterase 3A, cGMP-inhibited

Background

Hydrolyzes both cyclic AMP (cAMP) and cyclic GMP (cGMP).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PDE3A
• Entrez:
  • 5139(Human)
  • 54611(Mouse)
  • 50678(Rat)