PAX8 Antibody (SD0832)

Western Blot: PAX8 Antibody (SD0832) [NBP2-67793] - Western blot analysis of PAX8 on SKOV-3 cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rab

Immunocytochemistry/Immunofluorescence: PAX8 Antibody (SD0832) [NBP2-67793] - Usage of PAX8 antibody in ICC of HL60 cells (negative control)

Immunohistochemistry-Paraffin: PAX8 Antibody (SD0832) [NBP2-67793] - Analysis of paraffin-embedded mouse kidney tissue using anti-PAX8 antibody. Counter stained with hematoxylin.

Immunocytochemistry/Immunofluorescence: PAX8 Antibody (SD0832) [NBP2-67793] - Staining PAX8 in SKOV-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: PAX8 Antibody (SD0832) [NBP2-67793] - Usage of PAX8 antibody in ICC of SKOV3 cells (positive control)

Immunohistochemistry-Paraffin: PAX8 Antibody (SD0832) [NBP2-67793] - Analysis of paraffin-embedded human kidney tissue using anti-PAX8 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: PAX8 Antibody (SD0832) [NBP2-67793] - Analysis of paraffin-embedded human thyroid tissue using anti-PAX8 antibody. Counter stained with hematoxylin.

Immunohistochemistry: PAX8 Antibody (SD0832) [NBP2-67793] - Usage of PAX8 antibody in IHC of human kidney cells

Product Discontinued

PAX8 Antibody (SD0832) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Recombinant protein within Human PAX8 aa 260-450 / 450. (SwissProt: Q06710 Human; SwissProt: Q00288 Mouse; SwissProt: P51974 Rat)
• Localization: Nucleus.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: PAX8
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for PAX8 Antibody (SD0832)

• paired box 8
• paired box gene 8
• paired box protein Pax-8
• paired domain gene 8

Background

PAX8 is a member of the paired box (PAX) family of transcription factors, typically containing a paired box domain, an octapeptide, and a paired-type homeodomain. This family plays critical roles during fetal development and cancer growth. The specific function of the PAX8 is unknown but it may involve kidney cell differentiation, thyroid development, or thyroid dysgenesis. Alternative splicing in the gene by inclusion or exclusion of exons 7 and/or 8 has produced several known products but the biological significance of the variants is unknown. Several other splice variants have been proposed but the full nature of these products has not been described.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PAX8