PANK3 Antibody (OTI3C4) - Azide and BSA Free

Western Blot: PANK3 Antibody (OTI3C4) - Azide and BSA Free [NBP2-73244] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PANK3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PANK3.

Flow Cytometry: PANK3 Antibody (OTI3C4) - Azide and BSA Free [NBP2-73244] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-PANK3 antibody, and then analyzed by flow cytometry.

Flow Cytometry: PANK3 Antibody (OTI3C4) - Azide and BSA Free [NBP2-73244] - Analysis of Hela cells, using anti-PANK3 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Flow Cytometry: PANK3 Antibody (OTI3C4) - Azide and BSA Free [NBP2-73244] - Analysis of Jurkat cells, using anti-PANK3 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, CyTOF-ready
• Clone: OTI3C4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

PANK3 Antibody (OTI3C4) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse PANK3 Antibody (OTI3C4) - Azide and BSA Free (NBP2-03298) is a monoclonal antibody validated for use in WB and Flow. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human recombinant protein of human PANK3 (NP_078870) produced in HEK293T cell.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: PANK3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1:100
  • Western Blot 1:500
• Theoretical MW: 40.9 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for PANK3 Antibody (OTI3C4) - Azide and BSA Free

• DKFZp686O22247
• EC 2.7.1.33
• FLJ12899
• hPanK3
• MGC16863
• pantothenate kinase 3
• Pantothenic acid kinase 3

Background

PANK3 encodes a protein belonging to the pantothenate kinase family. Pantothenate kinase is a key regulatory enzyme in the biosynthesis of coenzyme A (CoA) in bacteria and mammalian cells. It catalyzes the first committed step in the universal biosynthetic pathway leading to CoA and is itself subject to regulation through feedback inhibition by CoA. This family member is expressed most abundantly in the liver. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PANK3