PAF49 Antibody (635) - Azide and BSA Free

Western Blot: PAF49 Antibody (635) [NBP2-43633] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PAF49 antibody diluted at 1:1000.

Immunocytochemistry/Immunofluorescence: PAF49 Antibody (635) [NBP2-43633] - Analysis of U2OS cells were fixed in 4% paraformaldehyde/PBS for 15 min. Green: CD3EAP protein stained by CD3EAP antibody diluted at 1:500. Blue: Hoechst 33342 staining.

Western Blot: PAF49 Antibody (635) [NBP2-43633] - Analysis of A. 30 ug 293T whole cell lysate/extract B. 30 ug A431 whole cell lysate/extract C. 30 ug HeLa whole cell lysate/extract D. 30 ug HepG2 whole cell lysate/extract E. 30 ug A375 whole cell lysate/extract 7.5 % SDS-PAGE PAF49 antibody [635] dilution: 1:1000.

Western Blot: PAF49 Antibody (635) [NBP2-43633] - Non-transfected (-) and transfected (+) 293T whole cell extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PAF49 antibody [635]. HRP-conjugated anti-mouset IgG antibody was used to detect the primary antibody.

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clone: 635
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

PAF49 Antibody (635) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse PAF49 Antibody (635) - Azide and BSA Free (NBP2-43633) is a monoclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human PAF49. The exact sequence is proprietary.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: CD3EAP
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 55 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein G purified

Alternate Names for PAF49 Antibody (635) - Azide and BSA Free

• A34.5
• antisense to ERCC 1
• Antisense to ERCC-1 protein
• ASE-1ASE1
• CASTMGC118851
• CD3 epsilon associated protein
• CD3e antigen, epsilon polypeptide associated protein
• CD3e molecule, epsilon associated protein
• CD3E-associated protein
• CD3-epsilon-associated protein
• DNA-directed RNA polymerase I subunit RPA34
• PAF49RNA polymerase I-associated factor PAF49

Background

PAF49/CAST is subunit of RNA polymerase I. PAF49/CAST has been shown to interact with activator upstream binding factor (UBF) and plays an important role in rDNA transcription.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CD3EAP