PACAP/ADCYAP1 Overexpression Lysate (Native)


Western Blot: ADCYAP1 Overexpression Lysate (Adult Normal) [NBL1-07649] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ADCYAP1.

Product Details

Applications WB
The exact concentration of the protein of interest cannot be determined for overexpression lysates. Please contact technical support for more information.

Order Details

PACAP/ADCYAP1 Overexpression Lysate (Native) Summary

The lysate was created in HEK293T cells, using plasmid ID RC202141 and based on accession number NM_001658. The protein contains a C-terminal DDK tag.
C-terminal Myc/DDK
Homo sapiens ADCYAP1 mRNA.

Packaging, Storage & Formulations

Store at -80C. Avoid freeze-thaw cycles.
RIPA buffer


  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
20.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Lysate Details for PACAP/ADCYAP1

Protein State


HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for PACAP/ADCYAP1 Overexpression Lysate (Native)

  • adenylate cyclase activating polypeptide 1 (pituitary)
  • PACAPMGC126852
  • pituitary adenylate cyclase-activating polypeptide


This gene encodes adenylate cyclase activating polypeptide 1. Mediated by adenylate cyclase activating polypeptide 1 receptors, this polypeptide stimulates adenylate cyclase and subsequently increases the cAMP level in target cells. Adenylate cyclase activating polypeptide 1 is not only a hypophysiotropic hormone, but also functions as a neurotransmitter and neuromodulator. In addition, it plays a role in paracrine and autocrine regulation of certain types of cells. This gene encodes three different mature peptides, including two isotypes, a shorter form and a longer form. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Contact Information

Product PDFs


Gene Symbol ADCYAP1

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