p38 beta/MAPK11 Antibody (280071) [Unconjugated]

p38 beta /MAPK11 was detected in immersion fixed Jurkat human acute T cell leukemia cell line using Mouse Anti-Human p38 beta /MAPK11 Monoclonal Antibody (Catalog # MAB3274) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

• Reactivity: Hu
• Applications: ICC/IF
• Clone: 280071
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

p38 beta/MAPK11 Antibody (280071) [Unconjugated] Summary

• Immunogen: Human p38 beta /MAPK11 synthetic peptide
  EPPKPPGSLEIEQ
  Accession # NP_002742
• Specificity: Detects human p38 beta /MAPK11 in direct ELISAs. No cross-reactivity with recombinant human p38 alpha, gamma, or delta is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: MAPK11
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 8-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for p38 beta/MAPK11 Antibody (280071) [Unconjugated]

• EC 2.7.11
• EC 2.7.11.24
• MAP kinase 11
• MAP kinase p38 beta
• MAPK 11
• MAPK11
• mitogen-activated protein kinase 11
• Mitogen-activated protein kinase p38 beta
• mitogen-activated protein kinase p38-2
• MPK11
• p38 beta
• p38b
• p38beta
• P38BETA2
• PRKM11
• SAPK2B
• SAPK2p38-2P38B
• Stress-activated protein kinase 2
• stress-activated protein kinase-2
• stress-activated protein kinase-2b

Background

The p38 mitogen-activated protein kinases (p38 MAP kinases) are a family of four related Ser/Thr kinases responsive to pro-inflammatory cytokines and environmental stresses, including ionizing radiation, oxidative stress, and osmotic shock. Each family member, p38 alpha , p38 beta , p38δ, and p38 gamma , is activated by dual Thr and Tyr phosphorylation within a Thr-Gly-Tyr motif residing in the kinase activation loop. For p38 beta , also known as p38-2, stress-activated protein kinase 2B (SAPK2B), and MAPK11, this dual phosphorylation occurs at Thr180/Tyr182.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MAPK11
• Uniprot:
  • NP_002742()