P2X3/P2RX3 Antibody

Western Blot: P2X3/P2RX3 Antibody [NBP2-19657] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with P2X3 antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunocytochemistry/Immunofluorescence: P2X3 Antibody [NBP2-19657] - Immunofluorescence analysis of methanol-fixed MCF-7, using antibody at 1:500 dilution.

Immunohistochemistry-Paraffin: P2X3 Antibody [NBP2-19657] - Immunohistochemical analysis of paraffin-embedded PC14 xenograft, using antibody at 1:500 dilution.

Western Blot: P2X3/P2RX3 Antibody [NBP2-19657] - Human tissue extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with P2X3 antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Product Discontinued

P2X3/P2RX3 Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human P2X3. The exact sequence is proprietary.
• Localization: Membrane; Multi-pass membrane protein
• Predicted Species: Rat (94%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: P2RX3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:1000-1:10000
• Theoretical MW: 44 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 20% Glycerol (pH7).
• Preservative: 0.025% Proclin 300
• Purity: Immunogen affinity purified

Alternate Names for P2X3/P2RX3 Antibody

• ATP receptor
• MGC129956
• P2RX3
• P2X purinoceptor 3
• P2X receptor, subunit 3
• P2X3
• P2X3purinergic receptor P2X3
• purinergic receptor P2X, ligand-gated ion channel, 3
• Purinergic receptor
• purinoceptor P2X3

Background

The product of this gene belongs to the family of purinoceptors for ATP. This receptor functions as a ligand-gated ion channel and may transduce ATP-evoked nociceptor activation. Mouse studies suggest that this receptor is important for peripheral pain responses, and also participates in pathways controlling urinary bladder volume reflexes. It is possible that the development of selective antagonists for this receptor may have a therapeutic potential in pain relief and in the treatment of disorders of urine storage. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: P2RX3
• Uniprot:
  • P56373(Human)