p18INK4c/CDKN2C Antibody (18P118 (DCS-118))

Western Blot: p18INK4c/CDKN2C Antibody (18P118 (same as DCS-118)) [NB120-3216] - Analysis of HeLa Cells.

Immunohistochemistry-Paraffin: p18INK4c/CDKN2C Antibody (18P118 (same as DCS-118)) [NB120-3216] - Human tonsil stained with p18 (NB120-3216) using peroxidase-conjugate and AEC chromogen. Note nuclear staining of cells.

Immunoprecipitation: p18INK4c/CDKN2C Antibody (18P118 (same as DCS-118)) [NB120-3216] - Analysis of native IP.

Product Discontinued

p18INK4c/CDKN2C Antibody (18P118 (DCS-118)) Summary

• Immunogen: Purified recombinant p18INK4c protein
• Localization: Nuclear. Cytoplasmic.
• Specificity: p18 (18P118 (same as DCS-118))
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CDKN2C
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Paraffin 1:50
  • Immunoprecipitation 1 - 2 ug/ml
  • Western Blot 1 - 2 ug/ml
• Application Notes: WB: Detects an approx. 18 kDa, which corresponds to the predicted molecular weight of p18 / INK4c.
• Theoretical MW: 18 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  HeLa Whole Cell Lysate
  Human Tonsil Whole Tissue Lysate (Adult Whole Normal)
  p18INK4c/CDKN2C Overexpression Lysate

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.4) and 0.2% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.4 mg/ml
• Purity: Protein G purified

Alternate Names for p18INK4c/CDKN2C Antibody (18P118 (DCS-118))

• CDK6 inhibitor p18
• CDKN2C
• CDKN6
• cyclin-dependent inhibitor
• cyclin-dependent kinase 4 inhibitor C
• Cyclin-dependent kinase 6 inhibitor
• cyclin-dependent kinase inhibitor 2C (p18, inhibits CDK4)
• INK4Ccyclin-dependent kinase 6 inhibitor p18
• p18
• p18INK4c
• p18-INK4C
• p18-INK6

Background

p18 INK4c is a specific inhibitor of cdk4 / cdk6 (interacts strongly with CDK6 and weakly with CDK4), and is expressed during G1 to S transition in the eukaryotic cell division cycle. It is maximally induced as cells enter S phase. Highest expression levels found in skeletal muscle. Also found in pancreas and heart.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for p18INK4c/CDKN2C Antibody (NB120-3216)(2)

Publications using NB120-3216

• Styring E, Seinen J, Dominguez-Valentin M et al. Key Roles for MYC, KIT and RET signaling in secondary angiosarcomas. Br. J. Cancer 2014-07-15 [PMID: 24983371] (IHC-P, Human)
  
  Details:
  p18 INK4c antibody used at dilution 1 : 25 for IHC-P on Human's primary angiosarcomas and secondary angiosarcomas.
• Gagrica S, Brookes S, Anderton E et al. Contrasting behavior of the p18INK4c and p16INK4a tumor suppressors in both replicative and oncogene-induced senescence. Cancer Research. 2011-11-11 [PMID: 22080569]

Bioinformatics

• Gene Symbol: CDKN2C
• Entrez:
  • 1031(Human)
  • 12580(Mouse)
• Uniprot:
  • P42773(Human)