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Recombinant Human p16INK4a/CDKN2A Protein

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SDS-Page: Human p16 Protein [NBP2-35199]

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, PAGE
Format
Carrier-Free
Concentration
LYOPH

Order Details

View Available Formulations

Recombinant Human p16INK4a/CDKN2A Protein Summary

Description
A single non-glycosylated polypeptide chain containing 156 amino acids corresponding to p16INK4a/CDKN2A Source: E. coli

Amino Acid Sequence: MEPAAGSSME PSADWLATAA ARGRVEEVRA LLEAGALPNA PNSYGRRPIQ VMMMGSARVA ELLLLHGAEP NCADPATLTR PVHDAAREGF LDTLVVLHRA GARLDVRDAW GRLPVDLAEE LGHRDVARYL RAAAGGTRGS NHARIDAAEG PSDIPD

Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
CDKN2A
Purity
>95%, by SDS-PAGE and HPLC
Endotoxin Note
Less than 1 EU/ug of p16INK4a/CDKN2A as determined by LAL method.

Applications/Dilutions

Dilutions
  • SDS-Page
  • Western Blot
Theoretical MW
16.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NBP2-35199 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
Buffer
Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.
Preservative
No Preservative
Concentration
LYOPH
Purity
>95%, by SDS-PAGE and HPLC
Reconstitution Instructions
Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at <-20C.

Notes

This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.

Alternate Names for Recombinant Human p16INK4a/CDKN2A Protein

  • ARF
  • CDK4 inhibitor p16-INK4
  • CDK4I
  • CDK4IP14ARF
  • CDKN2
  • CDKN2A
  • cell cycle negative regulator beta
  • CMM2P16-INK4A
  • Cyclin-dependent kinase 4 inhibitor A
  • cyclin-dependent kinase inhibitor 2A (melanoma, p16, inhibits CDK4)
  • cyclin-dependent kinase inhibitor 2A
  • INK4
  • INK4a
  • MLM
  • MLMP16INK4
  • MTS-1
  • MTS1P14
  • Multiple tumor suppressor 1
  • p14
  • p14ARF
  • p16
  • p16-INK4
  • p16INK4a
  • p16-INK4a
  • P19
  • p19ARF
  • TP16

Background

Cyclin-dependent kinase inhibitors (CDKIs) are proteins that bind to and inhibit the activity of CDKs. Two major classes of CDK inhibitors have been identified. The p16 family (p15, p16, p18 and p19) binds to and inhibits the activities of CDK4 and CDK6. The p21 family (p21, p27, p28 and p57) can bind to broad range of CDK-cyclin complexes and inhibit their activities. CDKIs are capable of suppressing growth, and several lines of evidence strongly suggest that at least some CDKIs may be tumor suppressor proteins. p16-INK4A is the member of p16 family and is encoded by CDKN2A gene in humans. It has three isoforms, which are wildly expressed but not detected in brain or skeletal muscle, except that isoform 3 is pancreas-specific. Defects in p16INK4A are a cause of Li-Fraumeni syndrome (LFS) and melanoma-astrocytoma syndrome (MASTS).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Publications for p16INK4a/CDKN2A Recombinant Protein (NBP2-35199)(3)

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for p16INK4a/CDKN2A Recombinant Protein (NBP2-35199). (Showing 1 - 1 of 1 FAQs).

  1. Our customer asked us about NBP1-02905, which we lately found it discontinued. While checking its related products, there seems none. Would you please help confirm if there is any replacement available?
    • With regards to your question on our CDKN2A antibodies, we do supply a wide range of products, but you haven't specified which application you are referring to. Please go through this link (cyclin-dependent kinase inhibitor 2A) and choose the product that will suit your experiment and the species you are working with. Meanwhile it is important to keep in mind that CDKN2A generates several transcript variants which differ in their first exons.

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Bioinformatics

Gene Symbol CDKN2A