p16INK4a/CDKN2A Antibody [Biotin]

• Reactivity: Hu
• Applications: WB, ELISA, IHC, KO
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Biotin

p16INK4a/CDKN2A Antibody [Biotin] Summary

• Immunogen: Antibody was raised against a peptide corresponding to 17 amino acids near the carboxy terminus of human CDKN2A. The immunogen is located within the last 50 amino acids of CDKN2A.
• Specificity: Human CDKN2A has 6 isoforms, including isoform 1 (156aa, 17kD), isoform 2 (105aa, 11kD), isoform 3 (116aa, 12kD), isoform 5 (167aa, 18kD), isoform tumor suppressor ARF (132aa, 14kD) and isoform smARF (85aa, 9kD). Mouse CDKN2A has 4 isoforms, including isoform 1 (168aa, 18kD), isoform 2 (125aa, 14kD), isoform tumor suppressor ARF (169aa, 19kD) and isoform smARF (125aa, 14kD). Rat CDKN2A has two isoforms, including isoform 1 (159aa, 17kD) and isoform ARF (160aa, 17kD). NBP3-06986 can detect only human isoforms 1 and 5, but cannot detect mouse and rat.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CDKN2A
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Knockout Validated
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

[Species Reactivity]

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Purity: Peptide affinity purified

Alternate Names for p16INK4a/CDKN2A Antibody [Biotin]

• ARF
• CDK4 inhibitor p16-INK4
• CDK4I
• CDK4IP14ARF
• CDKN2
• CDKN2A
• cell cycle negative regulator beta
• CMM2P16-INK4A
• Cyclin-dependent kinase 4 inhibitor A
• cyclin-dependent kinase inhibitor 2A (melanoma, p16, inhibits CDK4)
• cyclin-dependent kinase inhibitor 2A
• INK4
• INK4a
• MLM
• MLMP16INK4
• MTS-1
• MTS1P14
• Multiple tumor suppressor 1
• p14
• p14ARF
• p16
• p16-INK4
• p16INK4a
• p16-INK4a
• P19
• p19ARF
• TP16

Background

CDKN2A/p16INK4a is a gene that codes for a widely expressed protein with four isoforms, measuring 156, 105, 116, and 167 amino acids in length with weights of approximately 17, 11, 12, and 18 kDa respectively. CDKN2A/p16INK4a functions as a negative regulator of the proliferation of cells, which then allows them to interact with cyclins D and to phosphorylate the retinoblastoma protein. Current studies are being done on several diseases and disorders related to this gene including Li-Fraumeni syndrome, malignant peripheral nerve sheath tumor, blastic plasmacytoid dendritic cell, acth-secreting pituitary adenoma, recessive dystrophic epidermolysis bullosa, marginal zone b-cell lymphoma, adult astrocytic tumor, melanoma, and squamous cell carcinoma. CDKN2A/p16INK4a has also been shown to have interactions with MDM2, ZNF420, EGR1, MYCN, and CDK6 in pathways such as the cell cycle, p53 signaling, mitochondrial apoptosis, HIF-1 Alpha, CRHR, pancreatic cancer, and glioma pathways.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CDKN2A