p14ARF/CDKN2A Antibody (ARF 4C6/4)

Product Discontinued

p14ARF/CDKN2A Antibody (ARF 4C6/4) Summary

• Immunogen: His-tagged p14ARF protein expressed in bacteria.
• Localization: Nuclear
• Specificity: p14ARF (ARF 4C6/4)
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: CDKN2A
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 1:10-1:2000
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:100-1:2000
• Application Notes: ELISA, Immunofluorescence, Western Blot and Immunoprecipitation: In WB predicted molecular weight is 19 kDa. Optimal dilutions/concentrations should be determined by the end user.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein A purified

Alternate Names for p14ARF/CDKN2A Antibody (ARF 4C6/4)

• ARF
• CDK4 inhibitor p16-INK4
• CDK4IP14ARF
• CDKN2
• cell cycle negative regulator beta
• CMM2P16-INK4A
• Cyclin-dependent kinase 4 inhibitor A
• cyclin-dependent kinase inhibitor 2A (melanoma, p16, inhibits CDK4)
• cyclin-dependent kinase inhibitor 2A
• INK4
• INK4A
• MLMP16INK4
• MTS-1
• MTS1P14
• Multiple tumor suppressor 1
• p14
• p14ARF
• P16
• p16-INK4
• P16INK4A
• p16-INK4a
• P19
• P19ARF
• TP16

Background

The INK4a locus on chromosome 9p21 is frequently affected in human tumors. It encodes two structurally distinct tumor suppressor proteins, p16INK4a and the alternative reading frame protein, ARF (p14ARF in human and P19ARF in mouse). These two proteins interact with the upstream proteins: retinoblastoma protein and p53 tumor suppressors, respectively. Each of these proteins has a role in the senescence of primary cells, activates pathways for cell cycle control and tumor suppression. The p14ARF (or P19ARF in mouse) proteins can induce both G1 and G2 phase arrest in a manner that depends on functional p53. The mouse and human ARF proteins have different functions in tumor suppression; this distinction may contribute to the different levels of tumor proneness of these species.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CDKN2A
• Entrez:
  • 1029(Human)