p130Cas/BCAR1 [p Tyr410] Antibody - BSA Free

Western Blot: p130Cas/BCAR1 [p Tyr410] Antibody [NBP2-54638] - Western blot analysis of extracts from K562 cells (Lane 2) and 3T3 cells (Lane 3), using P130 Cas/BCAR1 [p Tyr410] Antibody. The lane on the left is treated with antigen-specific peptide.

Immunohistochemistry: p130Cas/BCAR1 [p Tyr410] Antibody [NBP2-54638] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using p130Cas/BCAR1 [p Tyr410] antibody (left) or the same antibody preincubated with blocking peptide (right).

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

p130Cas/BCAR1 [p Tyr410] Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit p130Cas/BCAR1 [p Tyr410] Antibody - BSA Free (NBP2-54638) is a polyclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: p130 Cas (phospho Tyr410) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 410 (G-V-Y (p)-A-V) derived from Human p130 Cas .
• Modification: p Tyr410
• Specificity: The antibody detects endogenous levels of p130Cas/BCAR1 only when phosphorylated at tyrosine 410.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: BCAR1
• Purity: Epitope affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:50-1:100
  • Western Blot 1:500-1:1000
• Theoretical MW: 130 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 °C.
• Buffer: PBS (pH 7.4) (without Mg2+ and Ca2+), 150mM NaCl, 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Epitope affinity purified

Alternate Names for p130Cas/BCAR1 [p Tyr410] Antibody - BSA Free

• BCAR1
• breast cancer estrogen resistance 1
• Cas scaffolding protein family member 1
• CAS1
• CASFLJ45059
• CASS1
• CASS1CRKASbreast cancer estrogen resistance protein 1
• CRKAS
• Crk-associated substrate p130Cas
• CRK-associated substrate
• FLJ12176
• p130Cas

Background

Docking protein which plays a central coordinating role for tyrosine kinase-based signaling related to cell adhesion. Implicated in induction of cell migration. Overexpression confers antiestrogen resistance on breast cancer cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: BCAR1