OCRL Antibody (S166A-26) - BSA Free

Western Blot: OCRL Antibody (S166A-26) [NBP2-42193] - analysis of Monkey COS cells transfected with GFP-tagged OCRL showing detection of 100 kDa INPP5F protein using Mouse Anti-INPP5F Monoclonal Antibody, Clone S166A-26 . Lane 1: Molecular Weight Ladder. Lane 2: Monkey COS cells transfected with GFP-tagged OCRL. Load: 15 ug . Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-INPP5F Monoclonal Antibody at 1:200 for 16 hours at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 100 kDa.

Immunocytochemistry/Immunofluorescence: OCRL Antibody (S166A-26) [NBP2-42193] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-INPP5F Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Cytoplasmic Vesicle, Endosome, Endosome Membrane, Membrane, Golgi Apparatus, Nucleus. Magnification: 60X.

Product Discontinued

OCRL Antibody (S166A-26) - BSA Free Summary

• Immunogen: Fusion protein amino acids 1-901 (full-length) of human INPP5F. Rat: 93% identity (845/904 amino acids identical). Mouse: 91% identity (824/901 amino acids identical) ~50% identity with INPP5b.
• Localization: Cytoplasmic Vesicle , Endosome , Endosome Membrane , Membrane , Golgi Apparatus
• Specificity: Approx 50% identity with INPP5b.Detects approx 100kDa. Cross-reacts with INPP5b.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: OCRL
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot 1:1000
• Application Notes: 1 ug/ml of INPP5F Antibody was sufficient for detection of INPP5F in 20 ug of COS cells transiently transformed with GFP-tagged OCLR lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary Antibody.

Reactivity Notes

Immunogen displays sequence identity for non-tested species: Mouse and Rat.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4), 50% Glycerol
• Preservative: 0.1% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for OCRL Antibody (S166A-26) - BSA Free

• EC 3.1.3.36
• INPP5F
• LOCR
• Lowe oculocerebrorenal syndrome protein
• NPHL2
• OCRL1inositol polyphosphate 5-phosphatase OCRL-1
• oculocerebrorenal syndrome of Lowe
• phosphatidylinositol polyphosphate 5-phosphatase

Background

OCRL encodes a phosphatase enzyme that is involved in actin polymerization and is found in the trans-Golgi network. Mutations in this gene cause oculocerebrorenal syndrome of Lowe and also Dent disease.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: OCRL
• Uniprot:
  • Q01968(Human)