| Reactivity | HuSpecies Glossary |
| Applications | PAGE |
| Concentration | 1 mg/ml |
| Description | A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-331 of Human NUDC Source: E.coli Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMGGEQEE ERFDGMLLAM AQQHEGGVQE LVNTFFSFLR RKTDFFIGGE EGMAEKLITQ TFSHHNQLAQ KTRREKRARQ EAERREKAER AARLAKEAKS ETSGPQIKEL TDEEAERLQL EIDQKKDAEN HEAQLKNGSL DSPGKQDTEE DEEEDEKDKG KLKPNLGNGA DLPNYRWTQT LSELDLAVPF CVNFRLKGKD MVVDIQRRHL RVGLKGQPAI IDGELYNEVK VEESSWLIED GKVVTVHLEK INKMEWWSRL VSSDPEINTK KINPENSKLS DLDSETRSMV EKMMYDQRQK SMGLPTSDEQ KKQEILKKFM DQHPEMDFSK AKFN |
| Source | E. coli |
| Protein/Peptide Type | Recombinant Protein |
| Gene | NUDC |
| Purity | >90%, by SDS-PAGE |
| Theoretical MW | 40.6 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | 20 mM Tris-HCl buffer (pH 8.0), 10% glycerol, 1 mM DTT |
| Preservative | No Preservative |
| Concentration | 1 mg/ml |
| Purity | >90%, by SDS-PAGE |
Diseases for NUDC Recombinant Protein (NBP2-52390)Discover more about diseases related to NUDC Recombinant Protein (NBP2-52390).
| Pathways for NUDC Recombinant Protein (NBP2-52390)View related products by pathway.
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | NUDC |
![SDS-Page: NUDC Recombinant Protein [NBP2-52390] - 15% SDS Page (3 ug)](http://images.novusbio.com/fullsize2/NUDC-Recombinant-Protein-SDS-Page-NBP2-52390-img0001.jpg "SDS-Page: NUDC Recombinant Protein [NBP2-52390] - 15% SDS Page (3 ug)")
![Western Blot: LIS1 Antibody [NBP1-87769] - Analysis of LIS1 in caco-2 whole cell lysate using anti-LIS1 antibody. Image submitted by a verified customer review.](http://images.novusbio.com/images2/LIS1-Antibody-Western-Blot-NBP1-87769-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: LIS1 Antibody [NBP1-87769] - Staining of human cell line U-251 MG shows localization to centrosome. Antibody staining is shown in green.](http://images.novusbio.com/images2/LIS1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87769-img0009.jpg)
![Immunohistochemistry-Paraffin: LIS1 Antibody [NBP1-87769] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/images2/LIS1-Antibody-Immunohistochemistry-Paraffin-NBP1-87769-img0014.jpg)
![Thrombopoietin/THPO [Unconjugated]](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "Thrombopoietin/THPO [Unconjugated]")
![Western Blot: PLK1 Antibody [NB100-56651] - Analysis of HCT-116 lysate using PLK1 antibody at 1:2000.](http://images.novusbio.com/images2/PLK1-Antibody-Western-Blot-NB100-56651-img0005.jpg)
![Immunohistochemistry-Paraffin: PLK1 Antibody [NB100-56651] - Analysis of human placenta using PLK1 antibody at 1:100.](http://images.novusbio.com/images2/PLK1-Antibody-Immunohistochemistry-Paraffin-NB100-56651-img0004.jpg)
![Western Blot: ERK1 Antibody (1E5) [NBP2-22203] - Western blot analysis of whole cell lysates from (1) MCF7 (2) NIH3T3 cell lines using ERK1 antibody (clone 1E5) at 1:1000 dilution. The signal was developed using HRP labeled goat-anti Mouse secondary antibody with ECL based detection.](http://images.novusbio.com/images2/ERK1-Antibody-(1E5)-Western-Blot-NBP2-22203-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: ERK1 Antibody (1E5) [NBP2-22203] - Analysis of NIH/3T3 cells using ERK1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/images2/ERK1-Antibody-(1E5)-Immunocytochemistry-Immunofluorescence-NBP2-22203-img0002.jpg)
![Immunohistochemistry-Paraffin: ERK1 Antibody (1E5) [NBP2-22203] - Immunohistochemical analysis of paraffin-embedded breast cancer tissues using ERK1 mouse mAb with DAB staining.](http://images.novusbio.com/images2/ERK1-Antibody-(1E5)-Immunohistochemistry-Paraffin-NBP2-22203-img0004.jpg)
![Immunohistochemistry-Paraffin: Integrin alpha 2b/CD41 Antibody [NBP1-84579] - Staining of human bone marrow.](http://images.novusbio.com/images2/Integrin-alpha-2b-CD41-Antibody-Immunohistochemistry-Paraffin-NBP1-84579-img0015.jpg)
![Immunohistochemistry-Paraffin: Integrin alpha 2b/CD41 Antibody [NBP1-84579] - Staining of human bone marrow shows high expression.](http://images.novusbio.com/images2/Integrin-alpha-2b-CD41-Antibody-Immunohistochemistry-Paraffin-NBP1-84579-img0005.jpg)
![Immunohistochemistry-Paraffin: Integrin alpha 2b/CD41 Antibody [NBP1-84579] - Staining of human stomach shows low expression as expected.](http://images.novusbio.com/images2/Integrin-alpha-2b-CD41-Antibody-Immunohistochemistry-Paraffin-NBP1-84579-img0006.jpg)
![Western Blot: Thyroid Peroxidase Antibody [NBP1-97765] - (0.3ug/ml) staining of Human Thyroid Gland lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/images/Thyroid Peroxidase Antibody-Western Blot-NBP1-97765-img0002.jpg)
![Immunohistochemistry-Paraffin: Thyroid Peroxidase Antibody [NBP1-97765] - Staining of paraffin embedded Human Thyroid Gland. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/images2/Thyroid-Peroxidase-Antibody-Immunohistochemistry-Paraffin-NBP1-97765-img0003.jpg)
![Western Blot: p23/PTGES3 Antibody (JJ3) [NB300-576] - Analysis of 25 ug of Hela (Lane 1), HepG2 (Lane 2), and mouse spleen cell lysates (Lane 3) and a molecular weight protein ladder.](http://images.novusbio.com/images2/p23-PTGES3 Antibody (JJ3)-Western Blot-NB300-576-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: p23/PTGES3 Antibody (JJ3) [NB300-576] - p23 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing p23 at a dilution of 1:500 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/images2/p23-PTGES3-Antibody-(JJ3)-Immunocytochemistry-Immunofluorescence-NB300-576-img0006.jpg)
![Immunohistochemistry-Paraffin: p23/PTGES3 Antibody (JJ3) [NB300-576] - Normal biopsies of deparaffinized Human testis tissue.](http://images.novusbio.com/images2/p23-PTGES3-Antibody-(JJ3)-Immunohistochemistry-Paraffin-NB300-576-img0003.jpg)
![Western Blot: Nudel Antibody (5E11) [NBP2-01086] Analysis of extracts (35ug) from 9 different cell lines by using anti-Nudel monoclonal antibody.](http://images.novusbio.com/images/Nudel Antibody (5E11)-Western Blot-NBP2-01086-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: Nudel Antibody (5E11) [NBP2-01086] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Nudel.](http://images.novusbio.com/images/Nudel Antibody (5E11)-Immunocytochemistry-Immunofluorescence-NBP2-01086-img0008.jpg)
![Immunohistochemistry-Paraffin: Nudel Antibody (5E11) [NBP2-01086] - Staining of paraffin-embedded Human Ovary tissue using anti-Nudel mouse monoclonal antibody.](http://images.novusbio.com/images/Nudel Antibody (5E11)-Immunohistochemistry-Paraffin-NBP2-01086-img0007.jpg)
![Knockdown Validated: Tmp21/p23 Antibody [NB110-57587] - Western blot shows lysates of HEK293T human embryonic kidney parental cell line and TMP21/TMED10 knockout (KO) HEK293T cell line. PVDF membrane was probed with 2.0 ug/ml of Rabbit Anti-Human TMP21/TMED10 Polyclonal Antibody (Catalog # NB110-57587) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for TMP21/TMED10 at approximately 25 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in the knockout HEK293T cell line. This experiment was conducted under reduc](http://images.novusbio.com/images2/Tmp21-p23-Antibody-Knockdown-Validated-NB110-57587-img0005.jpg)
![Western Blot: TMP21 Antibody [NB110-57587] - Detection of TMP21 in human liver lysate using NB110-57587.](http://images.novusbio.com/images2/TMP21 Antibody-Western Blot-NB110-57587-img0003.jpg)
![Immunohistochemistry-Paraffin: Tmp21/p23 Antibody [NB110-57587] - Tmp21/p23 was detected in immersion fixed paraffin-embedded sections of human liver using Rabbit Anti-Human TMP21/p23 polyclonal Antibody (Catalog # NB110-57587) at 1 ug/ml for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm in hepatocytes.](http://images.novusbio.com/images2/Tmp21-p23-Antibody-Immunohistochemistry-Paraffin-NB110-57587-img0004.jpg)
![Western Blot: NUDCD3 Antibody [NBP1-82939] - Analysis in human cell line RT-4 and human cell line U-251 MG.](http://images.novusbio.com/images2/NUDCD3-Antibody-Western-Blot-NBP1-82939-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: NUDCD3 Antibody [NBP1-82939] - Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane & cytosol.](http://images.novusbio.com/images2/NUDCD3-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-82939-img0006.jpg)
![Immunohistochemistry-Paraffin: NUDCD3 Antibody [NBP1-82939] - Staining of human liver.](http://images.novusbio.com/images2/NUDCD3-Antibody-Immunohistochemistry-Paraffin-NBP1-82939-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: CD34 Antibody (MEC 14.7) [NB600-1071] - CD34 antibody was tested in WEHI-3 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).](http://images.novusbio.com/images2/CD34 Antibody (MEC 14.7)-Immunocytochemistry-Immunofluorescence-NB600-1071-img0003.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody (MEC 14.7) [NB600-1071] - Analysis of a FFPE tissue section of mouse small intestine using rat anti-mouse CD34 (clone MEC 14.7) at 1:100 dilution. The signal was developed using HRP-conjugated anti-rat secondary with DAB reagent which followed counterstaining of nuclei using hematoxylin. This antibody specifically labelled the endothelial cells in blood vessels located primarily in the sub-mucosa, and of that of the mucosa muscularis and the mucosal lacteal.](http://images.novusbio.com/images2/CD34-Antibody-(MEC-14.7)-Immunohistochemistry-Paraffin-NB600-1071-img0014.jpg)
![Flow Cytometry: CD34 Antibody (MEC 14.7) [NB600-1071] - An intracellular stain was performed on Raw264.7 cells with CD34 Antibody [MEC 14.7] NB600-1071 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Rat IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 488 (SA5-10018, Thermo Fisher).](http://images.novusbio.com/images2/CD34-Antibody-(MEC-14.7)-Flow-Cytometry-NB600-1071-img0015.jpg)
![Immunohistochemistry-Paraffin: RRAS Antibody - Unpurified [NB100-56161] - RRAS was detected in immersion fixed paraffin-embedded sections of human placenta using this antibody at 1:500 for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm and plasma membrane in decidual and trophoblast cells.](http://images.novusbio.com/images2/RRAS-Antibody-Immunohistochemistry-Paraffin-NB100-56161-img0001.jpg)
![Western Blot: TPT1/TCTP Antibody (JU13-32) [NBP2-75686] - Analysis of TCTP on different lysates using anti-TCTP antibody at 1/1,000 dilution.Positive control:Lane 1: Mouse placenta tissue Lane 2: Mouse testis tissueLane 3: Hela Lane 4: A431 Lane 5: MCF-7](http://images.novusbio.com/images2/TPT1-TCTP Antibody (JU13-32)-Western Blot-NBP2-75686-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: TPT1/TCTP Antibody (JU13-32) [NBP2-75686] - Staining TCTP in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/images2/TPT1-TCTP-Antibody-(JU13-32)-Immunocytochemistry-Immunofluorescence-NBP2-75686-img0004.jpg)
![Immunohistochemistry-Paraffin: TPT1/TCTP Antibody (JU13-32) [NBP2-75686] - Analysis of paraffin-embedded mouse testis tissue using anti-TCTP antibody. Counter stained with hematoxylin.](http://images.novusbio.com/images2/TPT1-TCTP-Antibody-(JU13-32)-Immunohistochemistry-Paraffin-NBP2-75686-img0006.jpg)
![Western Blot: MKLP1 Antibody (SY02-74) [NBP2-67446] - Analysis of MKLP1 on different lysates using anti-MKLP1 antibody at 1/1,000 dilution. Positive control: Lane 1: A549 Lane 2: Hela](http://images.novusbio.com/images2/MKLP1 Antibody (SY02-74)-Western Blot-NBP2-67446-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: MKLP1 Antibody (SY02-74) [NBP2-67446] - Staining MKLP1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/images2/MKLP1-Antibody-(SY02-74)-Immunocytochemistry-Immunofluorescence-NBP2-67446-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: MKLP1 Antibody (SY02-74) [NBP2-67446] - Staining MKLP1 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/images2/MKLP1-Antibody-(SY02-74)-Immunocytochemistry-Immunofluorescence-NBP2-67446-img0001.jpg)
