Rat NTS2/NTSR2 ELISA Kit (Colorimetric)

ELISA: Rat NTS2/NTSR2 ELISA Kit (Colorimetric) [NBP2-76780] - Samples were spiked with high concentrations of Rat NTS2/NTSR2 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Rat NTS2/NTSR2 ELISA Kit (Colorimetric) [NBP2-76780] - Standard Curve Reference

• Reactivity: Rt
• Applications: ELISA
• Suitable Sample Type: Serum, plasma and other biological fluids
• Standard Curve Range: 0.313 - 20ng/mL
• Sensitivity: 0.188ng/mL

Rat NTS2/NTSR2 ELISA Kit (Colorimetric) Summary

• Standard Curve Range: 0.313 - 20ng/mL
• Sensitivity: 0.188ng/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: CV% < 5.7
• Intra-Assay: CV% < 5.63
• Spike Recovery: 84-109%
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: NTSR2

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Rat NTS2/NTSR2 ELISA Kit (Colorimetric)

• neurotensin receptor 2
• neurotensin receptor type 2
• neurotensin receptor, type 2
• NT-R-2
• NTR2Levocabastine-sensitive neurotensin receptor
• NTS2
• NTSR2

Background

Official Gene Symbol: NTSR2 Gen Bank Accession Number: NP_036476 Gene ID: 23620 (Human) Gene Map Locus: 2p25.1 (Human) NTR2 is a member of GPCR family. It contains a short N-terminal sequence, 7 transmembrane domains and a short cytoplasmic tail. It is a low affinity levocabastine-sensitive receptor for Neurotensin. Activation of NT2R by non-peptide agonists suggests that the receptor can couple to phospholipase C, phospholipase A2 and MAPK. Unlike NTR1, NTR2 is insensitive to GTP and has low sensitivity of sodium ions. Northern Blot analysis detected its expression in adult brain regions including the olfactory system, cerebral and crebellar cortices, hippocampus and hypothalamic nuclei. Its expression has also been found in low levels in kidney, uterus, heart and lung.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for NTS2/NTSR2 ELISA Kit (NBP2-76780). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: NTSR2