NRK1 Overexpression Lysate (Native)

Images

 
Western Blot: NRK1 Overexpression Lysate (Adult Normal) [NBL1-08607] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for NRK1.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

NRK1 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC200160 and based on accession number NM_017881. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens chromosome 9 open reading frame 95 (C9orf95), transcript variant 1, mRNA.
Gene
NMRK1

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
23 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for NRK1

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for NRK1 Overexpression Lysate (Native)

  • bA235O14.2
  • chromosome 9 open reading frame 95
  • EC 2.7.1.22
  • EC 2.7.1.n4
  • FLJ20559
  • nicotinamide riboside kinase 1
  • Nicotinic acid riboside kinase 1
  • NmR-K 1
  • NRK 1
  • NRK1
  • Ribosylnicotinamide kinase 1
  • Ribosylnicotinic acid kinase 1
  • RNK 1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for NRK1 Lysate (NBL1-08607). (Showing 1 - 1 of 1 FAQ).

  1. I am interested in using this product as an enzyme, and before ordering I would like to know if it is enzymatically active?
    • In regards to your inquiry about the lysates and protein we sell, unless otherwise noted on the datasheet these products are not considered active. The lysates will be transfected with the protein sequence and do not always undergo the same post translational modifications and folding as an endogenous protein. Unless specifically stated we cannot guarantee activity. These lysates are mainly intended as positive controls in Western Blot.

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Bioinformatics

Gene Symbol NMRK1