NRK1 Overexpression Lysate

Western Blot: NRK1 Overexpression Lysate (Adult Normal) [NBL1-08607] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for NRK1.

• Reactivity: Hu
• Applications: WB

NRK1 Overexpression Lysate Summary

• Description:

  NRK1 Transient Overexpression Lysate

  
  Expression Host: HEK293T
  
  Plasmid: RC200160
  
  Accession#: NM_017881
  
  Protein Tag: C-MYC/DDK
  
  You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). The 2xSDS Sample Buffer consists of 4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT.
• Gene: NMRK1

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: This product is intended for use as a positive control in Western Blot. Overexpression of the target protein was confirmed using an antibody to DDK (FLAG) epitope tag (NBP1-71705) present on the protein construct.
  
  Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading.
• Theoretical MW: 23 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: RIPA buffer

Lysate Details for Array

• Type: Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.

Alternate Names for NRK1 Overexpression Lysate

• bA235O14.2
• C9orf95
• cDNA FLJ39825
• EC 2.7.1.173
• EC 2.7.1.22
• EC 2.7.1.n4
• FLJ20559
• nicotinamide riboside kinase 1
• Nicotinic acid riboside kinase 1
• NmR-K 1
• NMRK1
• NRK 1
• NRK1
• Ribosylnicotinamide kinase 1
• Ribosylnicotinic acid kinase 1
• RNK 1

Background

Nicotinamide adenine dinucleotide (NAD+) is essential for life in all organisms, both as a coenzyme for oxidoreductases and as a source of ADP-ribosyl groups used in various reactions. Nicotinic acid and nicotinamide, collectively known as niacin, are the vitamin precursors of NAD+. Nicotinamide riboside kinases, such as NRK1, function to synthesize NAD+ through nicotinamide mononucleotide using nicotinamide riboside as the precursor (Bieganowski and Brenner, 2004 [PubMed 15137942]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

FAQs for NRK1 Lysate (NBL1-08607). (Showing 1 - 1 of 1 FAQ).

1. I am interested in using this product as an enzyme, and before ordering I would like to know if it is enzymatically active?
   • In regards to your inquiry about the lysates and protein we sell, unless otherwise noted on the datasheet these products are not considered active. The lysates will be transfected with the protein sequence and do not always undergo the same post translational modifications and folding as an endogenous protein. Unless specifically stated we cannot guarantee activity. These lysates are mainly intended as positive controls in Western Blot.

Bioinformatics

• Gene Symbol: NMRK1