NPRB/NPR2 Antibody (1060722) [Unconjugated]

HEK293 cells transfected with Human NPR2 and eGFP were stained with either (A) Mouse Anti-Human NPRB/NPR2 Monoclonal Antibody (Catalog # MAB9725) or (B) Mouse IgG2A Isotype Control (Catalog # MAB003) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow
• Clone: 1060722
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

NPRB/NPR2 Antibody (1060722) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line, NS0-derived human NPRB/NPR2
  Arg23-Ile458
  Accession # P20594
• Specificity: Detects human NPRB/NPR2 in direct ELISA.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from cell culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 0.25 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
  *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for NPRB/NPR2 Antibody (1060722) [Unconjugated]

• AMD1;AMDM;ANPb;ANPRB;Atrial natriuretic peptide receptor 2;ECDM;GCB;GC-B;GUC2B;GUCY2B;NPRB;NPRBi;SNSK
• AMDM
• ANPb
• ANPRB
• ECDM
• GC-B
• GUC2B
• NPR2
• NPRB
• NPRBi
• SNSK

Background

Human Natriuretic Peptide Receptor-2 (NPR2), also known as NPRB, ANP-RB or guanylyl Cyclase-B, is a member of the guanylyl cyclase family of proteins. NPR2 is a type I transmembrane glycoprotein that contains a 436 amino acid extracellular domain (ECD) (aa 23‑458) for ligand binding, and a 569 amino acid cytoplasmic domain that contains both a protein kinase domain and a carboxyl-terminal guanylate cyclase domain. NPR2 is expressed most highly in in bone, brain, fibroblasts, heart, kidney, liver, lung, uterine, and vascular smooth muscle tissue (1). NPR2 operates as an oligomer and binds both ANP (atrial natriuretic peptide) and BNP (B type natriuretic peptide), and NPR2 is the principal receptor of CNP (C type natriuretic peptide) (1, 2). Ligand binding to the extracellular ligand binding domain, plus ATP to the intracellular kinase domain activates a cytoplasmic guanylate cyclase (2). NPR2 pathway play a critical role in regulation of skeletal growth (3), and patients with single defect NPR2 alleles are statistically shorter than the average population (4). Over the extracellular domain, human NPR2 is 97% and 96% identical to mouse and rat NPR2, respectively.

1. Potter, L.R. et al. (2009) Handb Exp Pharmacol 191:341.
2. Chang, M.S. et al. (1989) Nature 341:68.
3. Tsuji, T. and Kunieda T. (2005) J Biol Chem 280:14288.
4. Olney, R.C. et al. (2006) J Clin Endocrinol Metab 91:1229.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • P20594()