non-muscle heavy chain 10 Myosin Antibody [CoraFluor™ 1]

CoraFluor™ 1, amine reactive (Catalog:7920) and CoraFluor™ 2, amine reactive (Catalog # 7950) are terbium-based probes that have been developed for use as TR-FRET donors. They emit wavelengths compatible with commonly used fluorescent acceptor dyes such as BODIPY® (or BDY) and Janelia Fluor® dyes, FITC (Catalog # 5440), TMR and Cyanine 5 (Catalog # 5436). CoraFluor™ fluorescence is brighter and more stable in biological media than existing TR-FRET donors, leading to enhanced sensitivity and improved data generation. CoraFluor™ 1 exhibits excitation upon exposure to a 337 nm UV laser.

• Reactivity: Hu
• Applications: IP, WB (-)
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: CoraFluor 1

non-muscle heavy chain 10 Myosin Antibody [CoraFluor™ 1] Summary

• Description: CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(TM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
  
  CoraFluor(TM) 1, amine reactive
  
  CoraFluor(TM) 1, thiol reactive
  
  For more information, please see our CoraFluor(TM) TR-FRET technology flyer.
• Immunogen: The immunogen recognized by this antibody maps to a region between residue 1935 and 1955 of human myosin, heavy chain 10, non-muscle using the numbering given in entry NP_005955.1 (GeneID 4628).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MYH10
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark. Do not freeze.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Immunogen affinity purified

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for non-muscle heavy chain 10 Myosin Antibody [CoraFluor™ 1]

• cellular myosin heavy chain, type B type B
• Cellular myosin heavy chain, type B
• heavy polypeptide 10, non-muscle
• MGC134913
• MGC134914
• MYH10
• Myosin heavy chain 10
• Myosin heavy chain, non-muscle IIb
• myosin heavy chain, nonmuscle type B
• myosin, heavy chain 10, non-muscle
• myosin-10
• near to the ATP binding region
• NMMHC II-b
• NMMHCB
• NMMHC-B
• NMMHC-IIB
• non-muscle heavy chain 10 Myosin
• Non-muscle myosin heavy chain B
• nonmuscle myosin heavy chain IIB
• Non-muscle myosin heavy chain IIb
• nonmuscle myosin heavy chain-B
• nonmuscle myosin II heavy chain-B

Background

Myosin II is a hexameric protein that consist of a pair of heavy chain subunits (MHC), a pair of essential light chain subunits (MLC), and a pair of regulatory light chain subunits (RLCs). Non-muscle myosin heavy chain II-B is encoded by the myosin heavy chain 10 gene (MYH10) and non-muscle myosin heavy chain II-A is encoded by the Myosin heavy chain 9 gene (MYH9). Myosin in non-muscle cells is involved in motile cellular processes which include cytokinesis, cell shape, secrection, and capping. Alternate names for Myosin-10 is Myosin heavy chain II-B, cellular myosin heavy chain type B, and NMMHCB.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MYH10