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![Immunohistochemistry-Paraffin: non-muscle heavy chain 10 Myosin Antibody [NBP2-38566] - Analysis in human placenta and skeletal muscle tissues. Corresponding MYH10 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/non-muscle-heavy-chain-10-Myosin-Antibody-Immunohistochemistry-Paraffin-NBP2-38566-img0019.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: non-muscle heavy chain 10 Myosin Antibody [NBP2-38566] - Analysis in human placenta and skeletal muscle tissues. Corresponding MYH10 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: non-muscle heavy chain 10 Myosin Antibody [NBP2-38566] - Analysis in human cell lines HEK293 and PC-3 using anti-MYH10 antibody. Corresponding MYH10 RNA-seq data are presented for the same cell lines. Loading control: anti-PFN1.](http://images.novusbio.com/fullsize/non-muscle-heavy-chain-10-Myosin-Antibody-Western-Blot-NBP2-38566-img0015.jpg)
![Immunohistochemistry-Paraffin: non-muscle heavy chain 10 Myosin Antibody [NBP2-38566] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/non-muscle-heavy-chain-10-Myosin-Antibody-Immunohistochemistry-Paraffin-NBP2-38566-img0021.jpg)
![Immunohistochemistry-Paraffin: non-muscle heavy chain 10 Myosin Antibody [NBP2-38566] - Staining of human placenta shows moderate membranous positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/non-muscle-heavy-chain-10-Myosin-Antibody-Immunohistochemistry-Paraffin-NBP2-38566-img0016.jpg)
![Immunohistochemistry-Paraffin: non-muscle heavy chain 10 Myosin Antibody [NBP2-38566] - Staining of human cerebellum shows weak to moderate cytoplasmic positivity in Purkinje cells.](http://images.novusbio.com/fullsize/non-muscle-heavy-chain-10-Myosin-Antibody-Immunohistochemistry-Paraffin-NBP2-38566-img0018.jpg)
![Immunohistochemistry-Paraffin: non-muscle heavy chain 10 Myosin Antibody [NBP2-38566] - Staining of human kidney shows moderate membranous positivity in cells in tubules.](http://images.novusbio.com/fullsize/non-muscle-heavy-chain-10-Myosin-Antibody-Immunohistochemistry-Paraffin-NBP2-38566-img0020.jpg)
![Immunohistochemistry-Paraffin: non-muscle heavy chain 10 Myosin Antibody [NBP2-38824] - Staining of human skeletal muscle shows low expression as expected.](http://images.novusbio.com/fullsize/non-muscle-heavy-chain-10-Myosin-Antibody-Immunohistochemistry-Paraffin-NBP2-38824-img0003.jpg "Immunohistochemistry-Paraffin: non-muscle heavy chain 10 Myosin Antibody [NBP2-38824] - Staining of human skeletal muscle shows low expression as expected.")
![Immunohistochemistry: non-muscle heavy chain 10 Myosin Antibody [NBP2-38824] - Staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells.](http://images.novusbio.com/fullsize/non-muscle-heavy-chain-10-Myosin-Antibody-Immunohistochemistry-NBP2-38824-img0001.jpg)
![Immunohistochemistry-Paraffin: non-muscle heavy chain 10 Myosin Antibody [NBP2-38824] - Staining of human placenta shows high expression.](http://images.novusbio.com/fullsize/non-muscle-heavy-chain-10-Myosin-Antibody-Immunohistochemistry-Paraffin-NBP2-38824-img0002.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: non-muscle heavy chain 10 Myosin Antibody [NBP2-38824] - Staining in human placenta and skeletal muscle tissues using anti-MYH10 antibody. Corresponding MYH10 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/non-muscle-heavy-chain-10-Myosin-Antibody-Immunohistochemistry-Paraffin-NBP2-38824-img0004.jpg)
![Western Blot: non-muscle heavy chain 10 Myosin Antibody [NBP1-18932] - Whole cell lysate from HeLa, 293T and mouse NIH3T3 cells. Myosin-10 was also immunoprecipitated by rabbit anti-Myosin-10 antibody NBP1-18931.](http://images.novusbio.com/fullsize/non-muscle-heavy-chain-10-Myosin-Antibody-Western-Blot-NBP1-18932-img0002.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: non-muscle heavy chain 10 Myosin Antibody [NBP1-18932] - Whole cell lysate from HeLa, 293T and mouse NIH3T3 cells. Myosin-10 was also immunoprecipitated by rabbit anti-Myosin-10 antibody NBP1-18931.")
![Western Blot: non-muscle heavy chain 10 Myosin Antibody [NBP3-35203] - Western blot analysis of various lysates using non-muscle heavy chain 10 Myosin Rabbit pAb at 1:3000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-35203_rabbit-non-muscle-heavy-chain-10-myosin-pab-101220241233813.jpg "Western Blot: non-muscle heavy chain 10 Myosin Antibody [NBP3-35203] - Western blot analysis of various lysates using non-muscle heavy chain 10 Myosin Rabbit pAb at 1:3000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.")
![Immunoprecipitation: non-muscle heavy chain 10 Myosin Antibody [NBP1-18931] - Detection of human Myosin-10 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Myosin-10 antibody NBP1-18931 used for IP at 3 ug/mg lysate. Myosin-10 was also immunoprecipitated by rabbit anti-Myosin-10 antibody NBP1-18932, which recognizes a downstream epitope. For blotting immunoprecipitated Myosin-10, NBP1-18931 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/non-muscle-heavy-chain-10-Myosin-Antibody-Immunoprecipitation-NBP1-18931-img0003.jpg "<b>Independent Antibodies Validation. </b>Immunoprecipitation: non-muscle heavy chain 10 Myosin Antibody [NBP1-18931] - Detection of human Myosin-10 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Myosin-10 antibody NBP1-18931 used for IP at 3 ug/mg lysate. Myosin-10 was also immunoprecipitated by rabbit anti-Myosin-10 antibody NBP1-18932, which recognizes a downstream epitope. For blotting immunoprecipitated Myosin-10, NBP1-18931 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![Western Blot: non-muscle heavy chain 10 Myosin Antibody (S05-6D8) [NBP3-19995] - Western blot detection of non-muscle heavy chain 10 Myosin in A549 (Lane 1), HL-60 (Lane 2), U251 (Lane 3), U87-MG (Lane 4) cell lysates using NBP3-19995 (1:1000 diluted). Predicted band size: 229kDa. Observed band size: 229kDa.](http://images.novusbio.com/fullsize/non-muscle-heavy-chain-10-Myosin-Antibody-S05-6D8-Western-Blot-NBP3-19995-img0001.jpg "Western Blot: non-muscle heavy chain 10 Myosin Antibody (S05-6D8) [NBP3-19995] - Western blot detection of non-muscle heavy chain 10 Myosin in A549 (Lane 1), HL-60 (Lane 2), U251 (Lane 3), U87-MG (Lane 4) cell lysates using NBP3-19995 (1:1000 diluted). Predicted band size: 229kDa. Observed band size: 229kDa.")
![ELISA: Human non-muscle heavy chain 10 Myosin - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-39535] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-39535_human-non-muscle-heavy-chain-10-myosin-r-t-u-elisa-kit-2110202415282689.png "ELISA: Human non-muscle heavy chain 10 Myosin - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-39535] - Standard Curve Reference")