Recombinant Human NMNAT-1 Protein

Product Discontinued

Recombinant Human NMNAT-1 Protein Summary

• Description: A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-279 of Human NMNAT-1
  
  

  Source: E.coli

  Amino Acid Sequence: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMENS EKTEVVLLAC GSFNPITNMH LRLFELAKDY MNGTGRYTVV KGIISPVGDA YKKKGLIPAY HRVIMAELAT KNSKWVEVDT WESLQKEWKE TLKVLRHHQE KLEASDCDHQ QNSPTLERPG RKRKWTETQD SSQKKSLEPK TKAVPKVKLL CGADLLESFA VPNLWKSEDI TQIVANYGLI CVTRAGNDAQ KFIYESDVLW KHRSNIHVVN EWIANDISST KIRRALRRGQ SIRYLVPDLV QEYIEKHNLY SSESEDRNAG VILAPLQRNT AEAKT

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: NMNAT1
• Purity: >95% pure by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 36 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH8.0), 20% glycerol, 0.1 M NaCl, 1 mM DTT, 1 mM EDTA
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: >95% pure by SDS-PAGE

Alternate Names for Recombinant Human NMNAT-1 Protein

• EC 2.7.7.1
• EC 2.7.7.18
• NaMN adenylyltransferase 1
• nicotinamide mononucleotide adenylyltransferase 1
• nicotinamide nucleotide adenylyltransferase 1
• nicotinamide nucleotide adenylyltransferase
• Nicotinate-nucleotide adenylyltransferase 1
• NMN adenylyltransferase 1
• NMNAT1
• NMNAT-1
• NMNATPNAT1
• PNAT1
• pyridine nucleotide adenylyltransferase 1

Background

NMNAT1, also known as NMNAT or PNAT1, is a central enzyme in NAD biosynthesis, catalyzing the condensation of nicotinamide mononucleotide (NMN) or nicotinic acid mononucleotide (NaMN) with the AMP moiety of ATP to form NAD or NaAD. It is widely expressed with high levels in skeletal muscle, heart, liver and kidney. This protein appears to have the ability to protect against axonal degeneration following mechanical or toxic insults. Recombinant human NMNAT1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

FAQs for NMNAT-1 Recombinant Protein (NBP1-45296). (Showing 1 - 1 of 1 FAQ).

1. I am interested in using this product as an enzyme, and before ordering I would like to know if it is enzymatically active?
   • In regards to your inquiry about the lysates and protein we sell, unless otherwise noted on the datasheet these products are not considered active. The lysates will be transfected with the protein sequence and do not always undergo the same post translational modifications and folding as an endogenous protein. Unless specifically stated we cannot guarantee activity. These lysates are mainly intended as positive controls in Western Blot.

Bioinformatics

• Gene Symbol: NMNAT1