Nicotinic Acetylcholine R alpha 7/CHRNA7 Antibody

Images

 
Immunohistochemistry-Paraffin: Nicotinic Acetylcholine R alpha 7/CHRNA7 Antibody [NBP1-52375] - Analysis of anti-CHRNA7 antibody with human hippocampus and isocortex. Image from verified customer review.
Immunohistochemistry-Paraffin: Nicotinic Acetylcholine R alpha 7/CHRNA7 Antibody [NBP1-52375] - Analysis of anti-CHRNA7 antibody with human brain, cortex at concentration 3 ug/ml.

Product Details

Summary
Reactivity Hu, Po, Bv, Ca, Ch, Eq, Pm, Mu, Rt, Ha, Ma-Op, RbSpecies Glossary
Applications IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

Nicotinic Acetylcholine R alpha 7/CHRNA7 Antibody Summary

Immunogen
Peptide with sequence KRPGEDKVRPACQHKQ, from the internal region of the protein sequence according to NP_000737.1.
Specificity
Human CHRNA7. This antibody also reacts with CHRNA7-FAM7A fusion isoform 1 (NP_647536.1) and isoform 2 (NP_683709.1).
Predicted Species
Mouse (94%), Rat (94%), Hamster (94%), Opossum (100%), Rabbit (94%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CHRNA7
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 3-6 ug/ml
  • Peptide ELISA 1:128000
Application Notes
IHC: NBP1-52375 was validated for use in IHC on a panel of 21 IHC-P (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity.
Reviewed Applications
Read 1 Review rated 5
using
NBP1-52375 in the following applications:

Publications
Read Publication using
NBP1-52375 in the following applications:

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Gorilla (100%), Gibbon (100%), Marmoset (88%), Xenopus (88%), Zebrafish (88%), Bat (81%).

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
TBS (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Nicotinic Acetylcholine R alpha 7/CHRNA7 Antibody

  • a7 nicotinic acetylcholine receptor
  • alpha 7 neuronal nicotinic acetylcholine receptor
  • alpha-7 nicotinic cholinergic receptor subunit
  • cholinergic receptor, nicotinic, alpha 7
  • cholinergic receptor, nicotinic, alpha polypeptide 7
  • CHRNA7
  • CHRNA7-2
  • NACHRA7
  • neuronal acetylcholine receptor protein, alpha-7 chain
  • neuronal acetylcholine receptor subunit alpha-7
  • Nicotinic Acetylcholine R alpha 7
  • Nicotinic Acetylcholine Ra 7

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Nicotinic Acetylcholine R alpha 7/CHRNA7 Antibody (NBP1-52375)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: IHC-P.


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Review for Nicotinic Acetylcholine R alpha 7/CHRNA7 Antibody (NBP1-52375) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP1-52375:
Filter by Applications
IHC-P
(1)
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Filter by Species
Human
(1)
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Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin Nicotinic Acetylcholine R alpha 7/CHRNA7 NBP1-52375
Enlarge
5
reviewed by:
Anonymous
IHC-P Human 04/26/2014
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Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedHuman brian tissue
SpeciesHuman

Blocking

Blocking Detailsskim milk 3% in PBs for 30 minutes

Primary Anitbody

Dilution RatioNicotinic Acetylcholine Receptor alpha 7 Antibody [NBP1-52375], 1:100, overnight at 4C

Secondary Antibody

Secondary DescriptionAnti-Goat, Mouse, Rabbit Immunoglobulins/biotinylated
Secondary Manufacturer Cat#Dako E0453
Secondary Concentration1:100 1h at room temperature

Details

Detection NotesStreptavidin-HRP (1:200, Dako P0397, 3 min RT) - DAB; or streptavindin-Alexa 488 (1:300, Invitrogen S32354, 1h RT)
Fixation DetailsFormalin fixed paraffin embedded tissue
Wash Description1xPBS, 3x5 minutes

Product General Protocols

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FAQs for Nicotinic Acetylcholine R alpha 7/CHRNA7 Antibody (NBP1-52375). (Showing 1 - 3 of 3 FAQ).

  1. I am doing research on Neuroscience and I would like to use some antibodies, especially in Western Blots, but I could do other assays: - alpha7 nicotinic cholinergic receptor; - parvalbumin; - calbindin D28K; The preferred reactivity is anti-human and anti-mouse. Do you have these antibodies? Are they in stock? And what about the prizes: How much are they? How much are the shipping costs? Could you make me any offer?
    • We currently have three alpha7 nicotinic cholingergic receptor antibodies available for purchase. Please see this list for our Parvalbumin antibodies. None are currently validated in mouse. I would like to introduce you to our Innovators Reward Program. We have an excelent calbinin D28K antibody that has been validated for use in human and mouse and WB. The pricing and availability of our products depends on your country.
  2. I am looking to buy antibodies against most of the nicotinic receptor subunits for WB and IHC application. Could you please indicate which of your antibodies have been used by other researchers and provide publications showing their use? Thanks.
    • A list of our antibodies directed against he nicotinic acetylcholine receptors can be found here. Any products with publications will be indicated on the search page and can be found under the publications tab on the individual product's datasheet. Furthermore, we guarantee all of our products for the applications and species list on the datasheet. This should help narrow down your search.
  3. Would you please tell me whether your antibody Nicotinic Acetylcholine R alpha 7/CHRNA7 Antibody (NBP1-52375) is suitable for detecting rat tissue which is PFA perfusion fixed frozen sections?
    • We have not tested this product on frozen sections yet but since it worked well in IHC-P, there is a great chance that it will work on IHC-Fr. Technically speaking, IHC-P protocol poses the target proteins/antigens to more harsh conditions/reagents compared to IHC-Fr, ICC-IF and FLOW protocols. And most of antibodies which work for IHC-P application works for IHC-Fr, ICC-IF and FLOW also. Therefore, I would highly encourage you to test this product in your experiments via our Innovator's Reward program. Innovators Reward

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Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Anonymous
04/26/2014
Application: IHC-P
Species: Human

Bioinformatics

Gene Symbol CHRNA7
Entrez
Uniprot