NFIB Antibody (1H1)

Immunohistochemistry: NFIB Antibody (1H1) [NBP2-76899] - Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-NFIB/NF1B2 antibody. Counter stained with hematoxylin.

Flow Cytometry: NFIB Antibody (1H1) [NBP2-76899] - Flow cytometric analysis of SH-SY5Y cells with NFIB/NF1B2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-mouse IgG was used as the secondary antibody.

Immunohistochemistry: NFIB Antibody (1H1) [NBP2-76899] - Immunohistochemical analysis of paraffin-embedded rat heart tissue using anti-NFIB/NF1B2 antibody. Counter stained with hematoxylin.

Immunohistochemistry: NFIB Antibody (1H1) [NBP2-76899] - Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-NFIB/NF1B2 antibody. Counter stained with hematoxylin.

Immunohistochemistry: NFIB Antibody (1H1) [NBP2-76899] - Immunohistochemical analysis of paraffin-embedded human prostate tissue using anti-NFIB/NF1B2 antibody. Counter stained with hematoxylin.

Immunohistochemistry: NFIB Antibody (1H1) [NBP2-76899] - Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-NFIB/NF1B2 antibody. Counter stained with hematoxylin.

Product Discontinued

NFIB Antibody (1H1) Summary

• Immunogen: Recombinant full length protein corresponding to human NFIB. (SwissProt: O00712 Human; SwissProt: P97863 Mouse)
• Localization: Nucleus.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: NFIB
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
• Theoretical MW: 47 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-questions.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH7.4), 0.2% BSA, 50% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for NFIB Antibody (1H1)

• CCAAT-box-binding transcription factor
• CTF
• HMGIC/NFIB
• NF-I/B
• NFI-B
• NFIB2
• NFIB3
• NFI-RED
• nuclear factor 1 B-type
• Nuclear factor 1/B
• nuclear factor I/BNF1-B
• TGGCA-binding protein

Background

Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NFIB