Neuromedin BR/NMBR Antibody (466505) [CoraFluor™ 1]

CoraFluor™ 1, amine reactive (Catalog:7920) and CoraFluor™ 2, amine reactive (Catalog # 7950) are terbium-based probes that have been developed for use as TR-FRET donors. They emit wavelengths compatible with commonly used fluorescent acceptor dyes such as BODIPY® (or BDY) and Janelia Fluor® dyes, FITC (Catalog # 5440), TMR and Cyanine 5 (Catalog # 5436). CoraFluor™ fluorescence is brighter and more stable in biological media than existing TR-FRET donors, leading to enhanced sensitivity and improved data generation. CoraFluor™ 1 exhibits excitation upon exposure to a 337 nm UV laser.

• Reactivity: Hu
• Applications: Flow, CyTOF-ready, Flow
• Clone: 466505
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: CoraFluor 1

Neuromedin BR/NMBR Antibody (466505) [CoraFluor™ 1] Summary

• Description: CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(TM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
  
  CoraFluor(TM) 1, amine reactive
  
  CoraFluor(TM) 1, thiol reactive
  
  For more information, please see our CoraFluor(TM) TR-FRET technology flyer.
• Immunogen: NS0 mouse myeloma cell line transfected with human NMBR
  Met1-Met390
  Accession # AAB27330
• Specificity: Detects human Neuromedin B R/NMBR. Stains human Neuromedin B R/NMBR transfectants but not irrelevant transfectants.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: NMBR
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry
  • Intracellular Staining by Flow Cytometry
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark. Do not freeze.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein A or G purified

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for Neuromedin BR/NMBR Antibody (466505) [CoraFluor™ 1]

• Neuromedin B R
• neuromedin B receptor
• Neuromedin BR
• neuromedin-B receptor
• Neuromedin-B-preferring bombesin receptor
• NMBR
• NMB-R

Background

The neuromedin B receptor, a Bombesin Receptor, activates phospholipase and protein kinase C and enhances calcium mobilization. It binds neuromedin B, a potent mitogen and growth factor for normal and neoplastic lung and for gastrointestinal epithelial tissues. Neuromedin B receptor expression has been reported in brain, particularly the olfactory bulb and thalamus, and in colon, liver, placenta, testis, spinal cord, and gastrointestinal tract. In addition, it has been shown to be present in cancer of the lung, ovary, and prostate. ESTs have been isolated from B-cell/lung/testis and brain libraries.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NMBR