Neurochondrin Antibody

Images

 
Western Blot: Neurochondrin Antibody [NBP1-60892] - 1:500 antibody dilution in DiluObuffer. Apparent MW is 90 KDa.
Immunohistochemistry: Neurochondrin Antibody [NBP1-60892] - Mouse Stratum Lucidum, CA3. 1:50 antibody dilution.
Western Blot: Neurochondrin Antibody [NBP1-60892] - Antibody dilution is 1:500 in antibody dilution buffer. MW is 88.5kDa.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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Catalog# & Conjugate Size Price

Neurochondrin Antibody Summary

Immunogen
Synthetic peptides corresponding to unique epitope on NORB with peptide sequcne corresponding to (EETASHYRMAALEQCLSEP). The NORB peptide was covalently modified post-synthetically to achieve desired antigenicity.
Specificity
This antibody detects a single band of approximately 88.5kDa in Positive control samples. The antibody also labels a minor 35kDa, the identity of this protein is not known.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NCDN
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot > 1:500
  • ELISA < 1:10000
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:100
  • Immunoprecipitation 1:200
Application Notes
Neurochondrin is ideal for WB and ELISA applications. The dilutions for this antibody is for reference only, investigators are expected to determine the optimal conditions for specific assay. WB: > 1:500; IMM & i.p pull-down assays: n.d; IHC and ELISA <1:10,000. Application of this antibody in protocols not listed here does not necessarily exclude its use in such procedures.
Control Peptide
Neurochondrin Protein (NBP1-62176)
Publications
Read Publications using NBP1-60892.

Reactivity Notes

Rat reactivity reported in scientific literature (PMID:25009471)

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris/Glycine buffer pH 7.5-7.9, stabilizing protein, and glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Neurochondrin Antibody

  • KIAA0607NCDN-1
  • NCDN-2
  • neurochondrin

Background

Norbin, also known as Neurochondrin, is a neuron-specific cytoplasmic protein involved in dendrite outgrowth. Overexpression of norbin in cultured Neuro 2a cells induced neurite-outgrowth (1). Norbin is located at rat chromosome 5q36. In addition to its involvement in dendrite outgrowth, Norbin is implicated in neural plasticity, chondrocyte differentiation, and early embryogenesis and in bone metabolism by inducing bone marrow cells to absorb bone matrix (2). Norbin is also an important endogenous modulator of metabotropic glutamate receptor 5 (mGluR5). It increases the cell surface localization of the receptor and positively regulates its signaling by physically interacting with the receptor (3). Norbin also acts as a signal molecule. It is a negative regulator of Ca2+/Calmodulin-dependent protein kinase II (CaMKII) phosphorylation and it is essential for the spatial learning process of the neurons (4). Recently it was shown that Norbin is a Diaphanous1 (Dia1) interacting protein, specifically binding to only the formin homology 3 (FH3). Furthermore, it was uncovered that the first 100 amino acids, but not the C-terminal region, of Norbin were responsible for its effect on neurite outgrowth (5). Norbin knockout mice showed a behavioral phenotype associated with a rodent model of schizophrenia and disruption of norbin gene resulted in epileptic seizure (3). Targeted disruption of Norbin gene resulted in embryonic lethality (6). It is also suggested that Norbin is involved in age and sex dependent brain function. Norbin is expressed in hippocampus, amygdala, septum, nucleus accumbens and dorsal striatum. It is approximately an 87.5kDa protein (729 amino acids). The NORB-selective antibodies were generated against purified Slingshot1 isoform1 (NORB) protein. NORB-antibodies are affinity purified over immobilized antigen based chromatography, and the purified immunoglobulins are stabilized in antibody stabilization buffer. Antibodies to NORB will label ~87.5kDa protein in Western blot positive control samples for NORB and several other tissues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Neurochondrin Antibody (NBP1-60892)(5)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol NCDN