MCHR1 Antibody (851047) [Unconjugated]

Western blot shows lysates of human brain (hypothalamus) tissue, mouse brain (thalamus/hypothalamus) tissue, and rat brain (no cortex) tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat MCHR1 Monoclonal Antibody (Catalog # MAB7938) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for MCHR1 at approximately 45-50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western lane view shows lysates of human brain (hypothalamus) tissue and mouse brain tissue, loaded at 0.5 mg/mL. A specific band was detected for MCHR1 at approximately 50-52 kDa (as indicated) using 50 µg/mL of Mouse Anti-Human/Mouse/Rat MCHR1 Monoclonal Antibody (Catalog # MAB7938) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Simple Western
• Clone: 851047
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

MCHR1 Antibody (851047) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human MCHR1
  Ser2-Pro113
  Accession # Q99705
• Specificity: Detects human MCHR1 in ELISAs. Detects human, mouse and rat MCHR1 in Western Blots
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: MCHR1
• Purity: Protein A or G purified from hybridoma culture supernatant
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Simple Western 50 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A or G purified from hybridoma culture supernatant
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MCHR1 Antibody (851047) [Unconjugated]

• G protein-coupled receptor 24
• GPR24
• GPR24SLC-1
• G-protein coupled receptor 24
• MCH receptor 1
• MCH1R
• MCH-1R
• MCH1RMCHR-1
• MCHR1
• MCH-R1
• melanin-concentrating hormone receptor 1
• MGC32129
• SLC1
• SLC1MCHR
• Somatostatin receptor-like protein

Background

MCHR1 (melanin-concentrating hormone receptor-1), also called GPR24 (G-protein coupled receptor 24) or SLC1 (somatostatin receptor-like 1), is an approximately 44 kDa, seven-transmembrane receptor for MHC. It is most highly expressed in the brain but is also present in peripheral tissues. Engagement of MHCR1 has effects on food intake, energy homeostasis, anxiety and depression, such that rodents treated with small molecule antagonists weigh less and show reduced anxiety and depression in model systems than untreated rodents. Human MCHR1 shares 74% amino acid (aa) sequence identity with mouse MCHR1 between aa 1-113, and 82% with rat MCHR1 between aa 71-113.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MCHR1
• Uniprot:
  • Q99705(Human)