Neurochondrin Antibody [FITC]

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: FITC

Neurochondrin Antibody [FITC] Summary

• Immunogen: Synthetic peptides corresponding to unique epitope on NORB with peptide sequcne corresponding to (EETASHYRMAALEQCLSEP). The NORB peptide was covalently modified post-synthetically to achieve desired antigenicity.
• Specificity: This antibody detects a single band of approximately 88.5kDa in Positive control samples. The antibody also labels a minor 35kDa, the identity of this protein is not known.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NCDN
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA < 1:10000
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot > 1:500
• Application Notes: This antibody detects a single band of approximately 88.5kDa in PC- NORB samples. The antibody also labels a minor 35kDa, the identity of this protein is not known. Antibody NORB -101AP is ideal for WB and ELISA applications, other applications have not been tested. These antibodies do not cross react to human lactoferrin, bovine lactoferrin or bovine serum albumin. The species cross reactivity for these antibodies have not been examined fully. The dilutions for this antibody is for reference only, investigators are expected to determine the optimal conditions for specific assay. WB: > 1:500; IMM & i.p pull-down assays: n.d; IHC n.d. ELISA <1:10,000. Application of this antibody in protocols not listed here does not necessarily exclude its use in such procedures.

Reactivity Notes

Rat reactivity reported in scientific literature (PMID:25009471)

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris/Glycine buffer, pH 7.4-7.8, HEPES,BSA 0.5%, glycerol 30%.
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for Neurochondrin Antibody [FITC]

• KIAA0607NCDN-1
• NCDN-2
• neurochondrin

Background

Norbin, also known as Neurochondrin, is a neuron-specific cytoplasmic protein involved in dendrite outgrowth. Overexpression of norbin in cultured Neuro 2a cells induced neurite-outgrowth (1). Norbin is located at rat chromosome 5q36. In addition to its involvement in dendrite outgrowth, Norbin is implicated in neural plasticity, chondrocyte differentiation, and early embryogenesis and in bone metabolism by inducing bone marrow cells to absorb bone matrix (2). Norbin is also an important endogenous modulator of metabotropic glutamate receptor 5 (mGluR5). It increases the cell surface localization of the receptor and positively regulates its signaling by physically interacting with the receptor (3). Norbin also acts as a signal molecule. It is a negative regulator of Ca2+/Calmodulin-dependent protein kinase II (CaMKII) phosphorylation and it is essential for the spatial learning process of the neurons (4). Recently it was shown that Norbin is a Diaphanous1 (Dia1) interacting protein, specifically binding to only the formin homology 3 (FH3). Furthermore, it was uncovered that the first 100 amino acids, but not the C-terminal region, of Norbin were responsible for its effect on neurite outgrowth (5). Norbin knockout mice showed a behavioral phenotype associated with a rodent model of schizophrenia and disruption of norbin gene resulted in epileptic seizure (3). Targeted disruption of Norbin gene resulted in embryonic lethality (6). It is also suggested that Norbin is involved in age and sex dependent brain function. Norbin is expressed in hippocampus, amygdala, septum, nucleus accumbens and dorsal striatum. It is approximately an 87.5kDa protein (729 amino acids). The NORB-selective antibodies were generated against purified Slingshot1 isoform1 (NORB) protein. NORB-antibodies are affinity purified over immobilized antigen based chromatography, and the purified immunoglobulins are stabilized in antibody stabilization buffer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Neurochondrin Antibody (NBP1-62175)(5)

Publications using NBP1-62175

• Wang H, Westin L, Nong Y, Birnbaum S, Bendor J, Brismar H, Nestler E, Aperia A, Flajolet M, Greengard P. Norbin is an endogenous regulator of metabotropic glutamate receptor 5 signaling. Science;326(5959):1554-7. 2009-12-11 [PMID: 20007903]
• Schwaibold EM, Brandt DT. Identification of Neurochondrin as a new interaction partner of the FH3 domain of the Diaphanous-related formin Dia1. Biochem Biophys Res Commun;373(3):366-72. 2008-08-29 [PMID: 18572016]
• Dateki M, Horii T, Kasuya Y et al. Neurochondrin negatively regulates CaMKII phosphorylation, and nervous system-specific gene disruption results in epileptic seizure. J Biol Chem 2005-05-27 [PMID: 15790563]
• Mochizuki R, Dateki M, Yanai K, Ishizuka Y, Amizuka N, Kawashima H, Koga Y, Ozawa H, Fukamizu A. Targeted disruption of the neurochondrin/norbin gene results in embryonic lethality. Biochem Biophys Res Commun;310(4):1219-26. 2003-10-31 [PMID: 14559245]
• Ishiduka Y, Mochizuki R, Yanai K, Takatsuka M, Nonomura T, Niida S, Horiguchi H, Maeda N, Fukamizu A. Induction of hydroxyapatite resorptive activity in bone marrow cell populations resistant to bafilomycin A1 by a factor with restricted expression to bone and brain, neurochondrin. Biochim Biophys Acta;1450(1):92-8. 1999-05-06 [PMID: 10231559]

Bioinformatics

• Gene Symbol: NCDN