NETO2 Antibody

NETO2 was immunoprecipitated from lysates (500 µg) of mouse brain tissue following incubation with 1‑2 µg/mL Goat Anti-Human/Mouse NETO2 Antigen Affinity-purified Polyclonal Antibody for 8 hours at 2‑8 °C. NETO2-antibody complexes were absorbed using Protein G (Sigma). Immunoprecipitated NETO2 was detected by Western blot using 1 µg/mL Goat Anti-Human/Mouse NETO2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3859). View our recommended buffer recipes for immunoprecipitation.

• Reactivity: Hu, Mu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

NETO2 Antibody Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human NETO2 isoform 1
  Ile23-Lys345
  Accession # Q8NC67
• Specificity: Detects human and mouse NETO2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 10% cross-reactivity with recombinant human NETO1 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: NETO2
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 1-2 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for NETO2 Antibody

• Brain-specific transmembrane protein containing 2 CUB and 1 LDL-receptor classA domains protein 2
• BTCL2
• FLJ10430
• FLJ90456
• NEOT2
• NEOT2FLJ14724
• NETO2
• neuropilin (NRP) and tolloid (TLL)-like 2
• neuropilin and tolloid-like protein 2

Background

NETO2 is a neurospecific type I transmembrane protein belonging to the NETO family of proteins that share homology with the axon guidance receptors, neuropilin and the developmentally important metalloproteinase Tolloid/BMP-1. NETO proteins have two CUB domains, an LDL-receptor class A domain in their extracellular region and a conserved FXNPXY-like domain in the cytoplasmic region. A splice variant of NETO2 that lacks the CUB domains and most of the LDL class A domain has been reported. It is likely that like NETO1, NETO2 may be a synaptic protein with neurological function. Human and mouse NETO2 share 98% amino acid sequence homology in their extracellular domains.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NETO2
• Uniprot:
  • Q8NC67()