NEK11 Antibody (OTI4H5)

Western Blot: NEK11 Antibody (4H5) [NBP2-02911] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NEK11 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NEK11.

Immunocytochemistry/Immunofluorescence: NEK11 Antibody (4H5) [NBP2-02911] Staining of COS7 cells transiently transfected by pCMV6-ENTRY NEK11.

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clone: OTI4H5
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.48 mg/ml

NEK11 Antibody (OTI4H5) Summary

• Description: Novus Biologicals Mouse NEK11 Antibody (OTI4H5) (NBP2-02911) is a monoclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human recombinant protein of human NEK11 (NP_079076) produced in HEK293T cell.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: NEK11
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Western Blot 1:2000
• Theoretical MW: 74 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 0.48 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for NEK11 Antibody (OTI4H5)

• EC 2.7.11
• EC 2.7.11.1
• FLJ23495
• Never in mitosis A-related kinase 11
• NIMA (never in mitosis gene a)- related kinase 11
• NimA-related protein kinase 11
• serine/threonine-protein kinase Nek11

Background

This gene encodes a member of the never in mitosis gene A family of kinases. The encoded protein localizes to the nucleoli, and may function with NEK2A in the S-phase checkpoint. The encoded protein appears to play roles in DNA replication and response to genotoxic stress. Alternatively spliced transcript variants have been described.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NEK11
• Entrez:
  • 79858(Human)