NDUFS3 Antibody

Western Blot: NDUFS3 Antibody [NBP1-51940] - (0.3ug/ml) staining of Human Frontal Cortex lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu, Bv
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

NDUFS3 Antibody Summary

• Immunogen: Peptide with sequence C-DTRPTVRPRNDVAHK corresponding to internal region according to NP_004542.1.
• Epitope: C-DTRPTVRPRNDVAHK
• Predicted Species: Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: NDUFS3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:4000
  • Western Blot 0.3 - 1 ug/ml
• Application Notes: WB: Approx. 28 kDa band observed in human brain (frontal cortex) lysates (calculated MW of 30.2 kDa band according to NP_004542.1).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for NDUFS3 Antibody

• CI-30
• CI-30kD
• complex I 30kDa subunit
• Complex I-30kD
• EC 1.6.5.3
• EC 1.6.99.3
• EC 1.6.99.5
• NADH dehydrogenase (ubiquinone) Fe-S protein 3 (30kD) (NADH-coenzyme Qreductase)
• NADH dehydrogenase (ubiquinone) Fe-S protein 3, 30kDa (NADH-coenzyme Qreductase)
• NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial
• NADH dehydrogenase-ubiquinone 30 kDa subunit
• NADH-ubiquinone oxidoreductase 30 kDa subunit

Background

The multisubunit NADH:ubiquinone oxidoreductase (complex I) is the first enzyme complex in the electron transport chain of mitochondria. The iron-sulfur protein (IP) fraction of complex I is made up of 7 subunits. See NDUFS1 (MIM 157655).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NDUFS3 Antibody (NBP1-51940)(1)

Publication using NBP1-51940

• Martinvalet D, Dykxhoorn DM, Ferrini R, Lieberman J. Granzyme A cleaves a mitochondrial complex I protein to initiate caspase-independent cell death. Cell 2008-02-01 [PMID: 18485875]

Bioinformatics

• Gene Symbol: NDUFS3
• Entrez:
  • 4722(Human)
• Uniprot:
  • NP_004542.1(Human)