NCKAP1 Antibody

Flow Cytometry: NCKAP1 Antibody [NBP2-26086] - Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Immunocytochemistry/Immunofluorescence: NCKAP1 Antibody [NBP2-26086] - Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Immunocytochemistry/Immunofluorescence: NCKAP1 Antibody [NBP2-26086] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

• Reactivity: Hu, Mu, Rt, Po, Bv, Ca
• Applications: WB, ELISA, Flow, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

NCKAP1 Antibody Summary

• Description: Please note, this antibody is considered to be "Innovators Grade". Innovators Grade antibodies have been validated for limited uses and require additional characterization for most new species/applications. These antibodies are directed against antigens that have limited or no commercial antibodies available for them, or for which there are limited expression data available to us. In an effort to encourage customer feedback, Novus has made these antibodies available through our Innovators Reward program. Under this program, Novus will provide you a 50% refund on the purchased product as well as a 50% discount on a future product of equal value in exchange for your new data. Please contact us at innovators@novusbio.com for more details.
• Immunogen: Peptide with sequence C-TPREYLTSHLEIR, from the internal region of the protein sequence according to NP_038464.1; NP_995314.1.
• Specificity: This antibody is expected to recognize both reported isoforms (NP_038464.1; NP_995314.1).
• Predicted Species: Mouse (100%), Rat (100%), Porcine (100%), Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: NCKAP1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 ug/ml
  • Immunocytochemistry/ Immunofluorescence 10 ug/ml
  • Peptide ELISA Detection limit 1:128000
  • Western Blot 1:100 - 1:2000
• Application Notes: WB: Preliminary experiments gave an approx 100kDa band in Human Cerebellum, Cerebral Cortex, Skin and Tonsil lysates after 0.5ug/ml antibody staining, and was successfully blocked by incubation with the immunizing peptide. Please note the calculated MWt is 129kDa according to NP_038464.1 and approx. 110-125kDa bands have been observed by other sources.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for NCKAP1 Antibody

• FLJ11291
• HEM2p125Nap1
• KIAA0587
• Membrane-associated protein HEM-2
• MGC8981
• NAP 1
• NAP125
• NAP1Nap1
• NCK-associated protein 1

Background

Is part of lamellipodial complex that controls Rac-dependent actin remodeling

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NCKAP1 Antibody (NBP2-26086)(1)

Publication using NBP2-26086

• Suzuki T, Nishiyama K, Yamamoto A et al. Molecular cloning of a novel apoptosis-related gene, human Nap1 (NCKAP1), and its possible relation to Alzheimer disease. Genomics 2000-01-15 [PMID: 10673335]

Bioinformatics

• Gene Symbol: NCKAP1
• Entrez:
  • 10787(Human)
  • 50884(Mouse)
  • 58823(Rat)