Nav1.7 Antibody (N68/6) - BSA Free

Western Blot: Nav1.7 Antibody (N68/6) [NBP2-12904] - Western Blot analysis of Hamster CHO cells showing detection of Nav1.7 Sodium Channel protein using Mouse Anti-Nav1.7 Sodium Channel Monoclonal Antibody, Clone N68/6 (NBP2-12904). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Nav1.7 Sodium Channel Monoclonal Antibody (NBP2-12904) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.

Immunocytochemistry/Immunofluorescence: Nav1.7 Antibody (N68/6) [NBP2-12904] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Nav1.7 Monoclonal Antibody, Clone N68/6 (NBP2-12904). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-Nav1.7 Monoclonal Antibody (NBP2-12904) at 1:100 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Nav1.7 Antibody (D) Composite.

Immunohistochemistry: Nav1.7 Antibody (N68/6) [NBP2-12904] - Immunohistochemistry analysis using Mouse Anti-Nav1.7 Sodium Channel Monoclonal Antibody, Clone N68/6 (NBP2-12904). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Nav1.7 Sodium Channel Monoclonal Antibody (NBP2-12904) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.

Immunocytochemistry/Immunofluorescence: Nav1.7 Antibody (N68/6) [NBP2-12904] - Human hippocampal sections using this antibody.

Immunohistochemistry: Nav1.7 Antibody (N68/6) [NBP2-12904] - Immunohistochemistry analysis using Mouse Anti-Nav1.7 Sodium Channel Monoclonal Antibody, Clone N68/6 (NBP2-12904). Tissue: Brain Slice. Species: Mouse. Fixation: Frozen sections. Primary Antibody: Mouse Anti-Nav1.7 Sodium Channel Monoclonal Antibody (NBP2-12904) at 1:1000. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown). Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain.

Immunohistochemistry: Nav1.7 Antibody (N68/6) [NBP2-12904] - Immunohistochemistry analysis using Mouse Anti-Nav1.7 Sodium Channel Monoclonal Antibody, Clone N68/6 (NBP2-12904). Tissue: hippocampus. Species: Human. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Nav1.7 Sodium Channel Monoclonal Antibody (NBP2-12904) at 1:1000 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.

• Reactivity: Hu, Mu, Rt, Ha
• Applications: WB, ICC/IF, IHC, IP, MA
• Clone: N68/6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Nav1.7 Antibody (N68/6) - BSA Free Summary

• Immunogen: Fusion protein amino acids 1751-1946 (C-terminus) of human Nav1.7
• Localization: Membrane , Synapse
• Specificity: Detects approx 230kDa. No cross-reactivity against other Nav channels.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: SCN9A
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:1000
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:1000
  • Immunoprecipitation
  • Microarray
  • Western Blot 1:1000
• Application Notes: 1 ug/ml of Nav1.7 Antibody was sufficient for detection of Nav1.7 in 10 ug of HEK-293 cell lysate transiently expressing Nav1.7 by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary Antibody.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4), 50% Glycerol
• Preservative: 0.09% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for Nav1.7 Antibody (N68/6) - BSA Free

• ETHA
• FEB3B
• hNE-Na
• Nav1.7
• NENA
• NE-NA
• NENAVoltage-gated sodium channel subunit alpha Nav1.7
• Neuroendocrine sodium channel
• Peripheral sodium channel 1
• PN1
• PN1hNE-Na
• SCN9A
• sodium channel protein type 9 subunit alpha
• Sodium channel protein type IX subunit alpha
• sodium channel, voltage-gated, type IX, alpha subunit
• voltage-gated sodium channel alpha subunit Nav1.7
• voltage-gated, type IX, alpha polypeptide

Background

1 ug/mL was sufficient for detection of Nav1.7 in 10ug of HEK-293 cell lysate transiently expressing Nav1.7 by colorimetric immunoblot analysis using Goat anti-mouse IgG: HRP as the secondary antibody.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Nav1.7 Antibody (NBP2-12904)(2)

Publications using NBP2-12904

• Hiranuma, M;Okuda, Y;Fujii, Y;Richard, JP;Watanabe, T; Characterization of human iPSC-derived sensory neurons and their functional assessment using multi electrode array Scientific reports 2024-03-12 [PMID: 38472288]
• Antico O, Ordureau A, Stevens M Et al. Global ubiquitylation analysis of mitochondria in primary neurons identifies endogenous Parkin targets following activation of PINK1 Science advances 2021-11-12 [PMID: 34767452] (WB, Mouse)

Bioinformatics

• Gene Symbol: SCN9A