NANOS1 Antibody

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Western Blot: NANOS1 Antibody [NB100-1781] - (0.3ug/ml) staining of Human Testis lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

NANOS1 Antibody Summary

Immunogen
Peptide with sequence C-SARDGPPGKKLR corresponding to C-Terminus according to NP_955631.1.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
NANOS1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 - 3 ug/ml
  • Peptide ELISA Detection limit 1:64000
Application Notes
WB: Approx. 35-37 kDa band observed in human testis lysates is reported in scientific literature (PMID: 14962983).
Positive Control
Testis Lysate (NB820-59266)
Publications
Read Publications using NB100-1781.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for NANOS1 Antibody

  • nanos homolog 1 (Drosophila)
  • nanos homolog 1
  • NOS-1
  • NOS1EC_Rep1a

Background

NANOS1 may regulate the translation of specific mRNAs by forming a complex with PUM2 that associates with the 3' untranslated regions (UTRs) of mRNA targets. Predominantly expressed in testis. Specifically expressed during germline development. In adult tissues, it is mainly expressed in spermatogonia, the stem cells of the germline. Also expressed during meiosis in spermatocytes. Not present in late, post-meiotic stage germ cells. Expressed in fetal ovaries, while it is weakly or not expressed in mature postmeiotic oocytes, suggesting that it may be expressed in premeiotic female germ cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for NANOS1 Antibody (NB100-1781)(4)

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Video Protocols

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol NANOS1
Entrez
Uniprot