NANOS1 Antibody

Western Blot: NANOS1 Antibody [NB100-1781] - Staining of Human Testis lysate (35 ug protein in RIPA buffer).Antibody at 0.3 ug/mL. Detected by chemiluminescence.

• Reactivity: Hu
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

NANOS1 Antibody Summary

• Immunogen: Peptide with sequence C-SARDGPPGKKLR corresponding to C-Terminus according to NP_955631.1.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: NANOS1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:64000
  • Western Blot 1 - 3 ug/mL
• Application Notes: WB: Approx. 35-37 kDa band observed in human testis lysates is reported in scientific literature (PMID: 14962983).
• Control:

  Human Testis Whole Tissue Lysate (Adult Whole Normal)

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for NANOS1 Antibody

• nanos homolog 1 (Drosophila)
• nanos homolog 1
• NOS-1
• NOS1EC_Rep1a

Background

NANOS1 may regulate the translation of specific mRNAs by forming a complex with PUM2 that associates with the 3' untranslated regions (UTRs) of mRNA targets. Predominantly expressed in testis. Specifically expressed during germline development. In adult tissues, it is mainly expressed in spermatogonia, the stem cells of the germline. Also expressed during meiosis in spermatocytes. Not present in late, post-meiotic stage germ cells. Expressed in fetal ovaries, while it is weakly or not expressed in mature postmeiotic oocytes, suggesting that it may be expressed in premeiotic female germ cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NANOS1 Antibody (NB100-1781)(5)

Publications using NB100-1781

• Calo E, Gu B, et al. Tissue-selective effects of nucleolar stress and rDNA damage in developmental disorders. Nature 2018-02-01 [PMID: 29364875]
• Flynn RA, Do BT, Rubin AJ et al. 7SK-BAF axis controls pervasive transcription at enhancers. Nat Struct Mol Biol 2016-03-01 [PMID: 26878240]
• Calo E, Flynn RA, Martin L et al. RNA helicase DDX21 coordinates transcription and ribosomal RNA processing. Nature 2015-02-12 [PMID: 25470060] (WB)
• Clark AT, Bodnar MS, Fox M et al. Spontaneous differentiation of germ cells from human embryonic stem cells in vitro. Hum Mol Genet 2004-04-01 [PMID: 14962983]
• Jaruzelska J, Kotecki M, Kusz K et al. Conservation of a Pumilio-Nanos complex from Drosophila germ plasm to human germ cells. Dev Genes Evol 2003-04-01 [PMID: 12690449]

Bioinformatics

• Gene Symbol: NANOS1
• Entrez:
  • 340719(Human)
• Uniprot:
  • NP_955631.1(Human)