Myosin 1B Antibody

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Western Blot: Myosin 1B Antibody [NBP1-97778] - (1ug/ml) Analysis of NIH3T3 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Concentration
0.5 mg/ml

Order Details

Myosin 1B Antibody Summary

Immunogen
Peptide with sequence C-RTVEAKQEKVSTT corresponding to internal region according to NP_036355.2.
Specificity
This antibody is expected to recognize both reported isoforms (NP_001123630.1; NP_036355.2). Reported variants represent identical protein: NP_001123630.1, NP_001155291.1.
Clonality
Polyclonal
Host
Goat
Gene
MYO1B
Purity
Immunogen affinity purified
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Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Conjugate
Unconjugated
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Western Blot 1 - 3 ug/ml
  • Immunohistochemistry 5 - 10 ug/ml
  • Peptide ELISA Detection limit 1:16000
Application Notes
WB: Approx. 120 kDa band observed in NIH3T3 lysates (calculated MW of 125 kDa band according to human NP_036355.2 and 128 kDa band according to mouse NP_034993.2). IHC-P: Human cerebral cortex shows cytoplasm staining in the neuronal bodies.
Publications
Read Publication using NBP1-97778.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Rat, Canine, Bovine.

Alternate Names for Myosin 1B Antibody

  • MMIa
  • MMI-alpha
  • MYH-1c
  • MYO1B variant protein
  • Myosin I alpha
  • myosin IB
  • myosin-I alpha
  • myosin-Ib
  • myr1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Myosin 1B Antibody (NBP1-97778)(1)


Showing Publication 1 - 1 of 1.
Publication using NBP1-97778 Applications Species
Laakso JM, Lewis JH, Shuman H, Ostap EM. Myosin I can act as a molecular force sensor. Science 2008 Jul 4 [PMID:18599791]

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for Myosin 1B Antibody (NBP1-97778) (0)

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Secondary Antibodies

 

Isotype Controls

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Contact Information

Product PDFs

Bioinformatics

Gene Symbol MYO1B
Entrez
Uniprot

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