Muscarinic Acetylcholine Receptor M3/CHRM3 Antibody (580011) [CoraFluor™ 1]

• Reactivity: Hu
• Applications: Flow, IHC, CyTOF-ready
• Clone: 580011
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: CoraFluor 1

Muscarinic Acetylcholine Receptor M3/CHRM3 Antibody (580011) [CoraFluor™ 1] Summary

• Description: CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(TM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
  
  CoraFluor(TM) 1, amine reactive
  
  CoraFluor(TM) 1, thiol reactive
  
  For more information, please see our CoraFluor(TM) TR-FRET technology flyer.
• Immunogen: NS0 mouse myeloma cell line transfected with human CHRM3
  Accession # P20309
• Specificity: Detects HEK293 cells transfected with human CHRM3 in Cell-based ELISA
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: CHRM3
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry
  • Immunohistochemistry
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark. Do not freeze.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein A or G purified

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for Muscarinic Acetylcholine Receptor M3/CHRM3 Antibody (580011) [CoraFluor™ 1]

• cholinergic receptor, muscarinic 3
• CHRM3
• HM3
• m3 muscarinic receptor
• mAChR M3
• muscarinic acetylcholine receptor M3

Background

The muscarinic cholinergic receptors belong to a larger family of G protein-coupled receptors. The functional diversity of these receptors is defined by the binding of acetylcholine and includes cellular responses such as adenylate cyclase inhibition, phosphoinositide degeneration, and potassium channel mediation. Muscarinic receptors influence many effects of acetylcholine in the central and peripheral nervous system. The muscarinic cholinergic receptor 3 controls smooth muscle contraction and its stimulation causes secretion of glandular tissue.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CHRM3