Muscarinic Acetylcholine Receptor M2/CHRM2 Antibody (31-1D1)

Immunohistochemistry-Paraffin: Muscarinic Acetylcholine Receptor M2/CHRM2 Antibody (31-1D1) [NB120-2805] - Human kidney tissue.

• Reactivity: Hu, Mu, Rt, Po
• Applications: WB, Flow, ICC/IF, IHC, IP
• Clone: 31-1D1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 2 mg/ml

Muscarinic Acetylcholine Receptor M2/CHRM2 Antibody (31-1D1) Summary

• Description: Novus Biologicals Mouse Muscarinic Acetylcholine Receptor M2/CHRM2 Antibody (31-1D1) (NB120-2805) is a monoclonal antibody validated for use in IHC, WB, Flow, ICC/IF and IP. Anti-Muscarinic Acetylcholine Receptor M2/CHRM2 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Purified porcine cardiac m2 muscarinic acetylcholine receptor.
• Localization: Plasma membrane
• Specificity: This is specific for the m2 mAChR subtype.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CHRM2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:10 - 1:1000
  • Immunocytochemistry/ Immunofluorescence 2 ug/ml
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Paraffin 1:20
  • Immunoprecipitation 1:10 - 1:500
  • Western Blot 1:1000
• Application Notes: WB: Detects 60 - 80 kDa protein(s) (depending on the degree of glycosylation) representing the m2AChR / CHRM2.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS with 1 mg/ml BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 2 mg/ml
• Purity: Protein A purified

Alternate Names for Muscarinic Acetylcholine Receptor M2/CHRM2 Antibody (31-1D1)

• 7TM receptor
• cholinergic receptor, muscarinic 2
• CHRM2
• FLJ43243
• HM2
• mAChR M2
• MGC120006
• MGC120007
• muscarinic acetylcholine receptor M2
• muscarinic M2 receptor

Background

Acetylcholine receptors (AChRs) mediate synaptic transmission at the neuromuscular junction. The channel-linked AChR that mediates rapid, excitatory actions of acetylcholine is called nicotinic AChR (nAChR) because it can be activated by nicotine. The non-channel linked AChR that medicates the slow actions of acetylcholine, which can be either inhibitory or excitatory, is called muscarinic AChR (mAChR) because it can be activated by muscarine. The mAChRs are present in neurons of the central and peripheral nervous systems, cardiac and smooth muscle and various exocrine glands. There are 5 subtypes (m1-m5) of the receptor that have a tissue specific pattern of expression. The m2 receptor is localized primarily in cardiac tissue and is also expressed at low levels in the hippocampus, cortex, striatum, thalamus, basal forebrain, brainstem, lung, vas deferens and uterus.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Muscarinic Acetylcholine Receptor M2/CHRM2 Antibody (NB120-2805)(1)

Publication using NB120-2805

• Chiang BJ, Mao SH, Chung SD, Chien CT Adipose-derived stem cell-derived microvesicles prevent progression to bladder underactivity secondary to long-termed bilateral partial iliac arterial occlusion-induced bladder ischemia Research Square 2021-01-01 [PMID: 34210091] (WB, Rat)

Bioinformatics

• Gene Symbol: CHRM2
• Entrez:
  • 1129(Human)
  • 243764(Mouse)
  • 81645(Rat)