MT-ND4 Antibody - BSA Free

Western Blot: MT-ND4 Antibody [NBP2-47365] - Analysis in human cell line HeLa.

Immunohistochemistry-Paraffin: MT-ND4 Antibody [NBP2-47365] - Staining of human Stomach shows strong granular cytoplasmic positivity in enteroendocrine cells.

Immunohistochemistry-Paraffin: MT-ND4 Antibody [NBP2-47365] - Staining of human heart muscle shows moderate cytoplasmic positivity in myocytes.

Immunohistochemistry-Paraffin: MT-ND4 Antibody [NBP2-47365] - Staining of human Lymph node shows very weak granular cytoplasmic positivity in non-germinal center cells.

Immunohistochemistry-Paraffin: MT-ND4 Antibody [NBP2-47365] - Staining of human Salivary gland shows moderate granular cytoplasmic positivity in glandular cells.

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

MT-ND4 Antibody - BSA Free Summary

• Immunogen: This antibody was developed against a recombinant protein corresponding to amino acids: LANSNYERTHSRIIILSQGLQT
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ND4
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:20 - 1:50
  • Immunohistochemistry-Paraffin 1:20 - 1:50
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.
• Control Peptide:

  MT-ND4 Recombinant Protein Antigen (NBP2-47365PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for MT-ND4 Antibody - BSA Free

• ND4

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MT-ND4 Antibody (NBP2-47365)(6)

Publications using NBP2-47365

• Meng F, Zhou M, Xiao Y, et al. A deafness-associated tRNA mutation caused pleiotropic effects on the m1G37 modification, processing, stability and aminoacylation of tRNAIle and mitochondrial translation Nucleic acids research 2021-01-25 [PMID: 33398350] (WB, Human)
• Fan W, Zheng J, Kong W et al. Contribution of a mitochondrial tyrosyl-tRNA synthetase mutation to the phenotypic expression of the deafness-associated tRNASer(UCN) 7511AG mutation J. Biol. Chem. 2019-11-04 [PMID: 31685661] (WB, Human)
• Zhao X, Cui L, Xiao Y et al. Hypertension-associated mitochondrial DNA 4401A>G mutation caused the aberrant processing of tRNAMet, all 8 tRNAs and ND6 mRNA in the light-strand transcript Nucleic Acids Research 2019-08-28 [PMID: 31504769] (WB, Human)
• Jia Z, Meng F, Chen H et al. Human TRUB1 is a highly conserved pseudouridine synthase responsible for the formation of psi 55 in mitochondrial tRNAAsn, tRNAGln, tRNAGlu and tRNAPro Nucleic acids research 2022-08-26 [PMID: 36018806] (WB, Human)
• Igelmann S, Lessard F, Uchenunu O et al. A hydride transfer complex reprograms NAD metabolism and bypasses senescence Molecular cell 2021-09-16 [PMID: 34547241]

Bioinformatics

• Gene Symbol: ND4